RRC ID 60199
著者 Muangham S, Lipun K, Thamchaipenet A, Matsumoto A, Duangmal K.
タイトル Gordonia oryzae sp. nov., isolated from rice plant stems (Oryza sativa L.).
ジャーナル Int J Syst Evol Microbiol
Abstract A novel endophytic actinomycete, designated strain RS15-1ST, was isolated from surface-sterilized stems of Oryza sativa L. collected from Sisaket province, Thailand. The colony of strain was strong orange, catalase-positive and oxidase-negative. Growth occurred at a temperature range of 17-37 °C, at pH 4.0-9.0 and in the presence of 0-13 % (w/v) NaCl. Phylogenetic analyses based on the 16S rRNA sequences showed that strain RS15-1ST belonged to the genus Gordonia and was closely related to Gordonia polyisoprenivorans DSM 44302T (98.8 %) and Gordonia rhizosphera DSM 44383T (98.4 %). The major cellular fatty acids were C16 : 0, C18 : 0 10-methyl (tbsa), C16 : 1ω7c/C16 : 1ω6c and C18 : 1ω9c. The menaquinones were MK-9(H2) and MK-8(H2). Strain RS15-1ST contained meso-diaminopimelic acid, arabinose, galactose, mannose and ribose in whole-cell hydrolysates. The polar lipids of the strain were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol mannosides, an unidentified polar lipid and two unidentified phospholipids. The DNA G+C content was 66.3 mol%. In silico DNA-DNA hybridization of strain RS15-1ST showed 48.3 and 20.5 % relatedness to its closest neighbours, Gordonia polyisoprenivorans DSM 44302T and Gordonia rhizosphera DSM 44383T, respectively. Based on data of genotypic, phenotypic, phylogenetic and chemotaxonomic analysis, strain RS15-1ST represents a novel species of the genus Gordonia, for which the name Gordonia oryzae sp. nov. is proposed. The type strain is RS15-1ST (=TBRC 8485T=NBRC 113446T).
巻・号 69(6)
ページ 1621-1627
公開日 2019-6-1
DOI 10.1099/ijsem.0.003368
PMID 30932808
MeSH Bacterial Typing Techniques Base Composition DNA, Bacterial / genetics Diaminopimelic Acid / chemistry Fatty Acids / chemistry Gordonia Bacterium / classification* Gordonia Bacterium / isolation & purification Nucleic Acid Hybridization Oryza / microbiology* Phospholipids / chemistry Phylogeny* Pigmentation Plant Stems / microbiology* RNA, Ribosomal, 16S / genetics Sequence Analysis, DNA Thailand Vitamin K 2 / chemistry
IF 2.166
引用数 0
リソース情報
一般微生物 JCM10675 JCM10426