| RRC ID |
60200
|
| Author |
Barreteau H, Vandervennet M, Guédon L, Point V, Canaan S, Rebuffat S, Peduzzi J, Carré-Mlouka A.
|
| Title |
Haloarcula sebkhae sp. nov., an extremely halophilic archaeon from Algerian hypersaline environment.
|
| Journal |
Int J Syst Evol Microbiol
|
| Abstract |
A halophilic organism, SWO25T, was isolated from water sampled in Algeria at the salt lake (sebkha) of Ouargla. The novel strain stained Gram-negative, and cells were pleomorphic with a red pigmentation. Strain SWO25T grew optimally at 35-45 °C, at pH 6.0-8.0 and 0.05-0.25 M MgCl2 concentrations. Cells were extremely halophilic, with optimal growth at 4.3-5.1 M NaCl. The predominant membrane polar lipids were C20C20 glycerol diether derivatives of phosphatidylglycerol, phosphatidylglycerol phosphate, phosphatidylglycerol sulfate, triglycosyl diether and diglycosyl diether. The major respiratory menaquinone component was MK-8. Cells were highly tolerant to the presence of decane and isooctane in the growth medium. Chemotaxonomic properties supported the assignment of strain SWO25T to the genus Haloarcula. The DNA G+C content was 61.1mol%. DNA-DNA hybridization and phylogenetic analyses of the 16S rRNA and rpoB' genes showed that strain SWO25T is distinct from known Haloarcula species. Based on phenotypic, chemotaxonomic, genotypic and phylogenetic data, we describe a novel species of the genus Haloarcula, for which the name Haloarculasebkhae sp. nov. is proposed. The type strain is SWO25T (=CIP 110583T=JCM 19018T).
|
| Volume |
69(3)
|
| Pages |
732-738
|
| Published |
2019-3-1
|
| DOI |
10.1099/ijsem.0.003211
|
| PMID |
30628879
|
| MeSH |
Algeria
Base Composition
DNA, Archaeal / genetics
Fatty Acids / chemistry
Haloarcula / classification*
Haloarcula / isolation & purification
Lakes / microbiology*
Nucleic Acid Hybridization
Phospholipids / chemistry
Phylogeny*
Pigmentation
RNA, Ribosomal, 16S / genetics
Saline Waters*
Sequence Analysis, DNA
Vitamin K 2 / analogs & derivatives
Vitamin K 2 / chemistry
|
| IF |
2.166
|
| Times Cited |
0
|
| Resource |
| General Microbes |
JCM19018
JCM11927
JCM15759
JCM13557
JCM15760 |
