| Abstract |
A Gram-stain-positive, aerobic actinomycete strain, designated NEAU-LLET, was isolated from cow dung collected from Shangzhi, Heilongjiang Province, north-east China and characterized using a polyphasic approach. Phylogenetic analysis based on 16S rRNA gene sequence indicated that strain NEAU-LLET belonged to the genus Microbacterium, with highest sequence similarity to Microbacterium pseudoresistens DSM 22185T (98.2 %). The predominant menaquinones were MK-13 and MK-14. The major polar lipids were diphosphatidylglycerol, phosphatidylglycerol and a glycolipid. The major fatty acids were identified as anteiso-C15 : 0, iso-C16 : 0 and iso-C17 : 0. However, DNA-DNA relatedness and physiological and biochemical data showed that strain NEAU-LLET could be distinguished from its closest relative. Therefore, strain NEAU-LLET represents a novel species of the genus Microbacterium, for which the name Microbacteriumbovistercoris sp. nov is proposed, with NEAU-LLET (=CCTCC AA 2018025T=JCM 32662T) as the type strain.
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