RRC ID |
60287
|
著者 |
Takusagawa S, Satoh Y, Ohtsu I, Dairi T.
|
タイトル |
Ergothioneine production with Aspergillus oryzae.
|
ジャーナル |
Biosci Biotechnol Biochem
|
Abstract |
To establish a reliable and practical ergothioneine (ERG) supply, we employed fermentative ERG production using Aspergillus oryzae, a fungus used for food production. We heterologously overexpressed the egt-1 and -2 genes of Neurospora crassa in A. oryzae and succeeded in producing ERG (231.0 mg/kg of media, which was 20 times higher than the wild type). Abbreviations: ERG: ergothioneine; HER: hercynine; Cys-HER: hercynylcysteine-sulfoxide; SAM: S-adenosylmethionine; SAH: S-adenosylhomocysteine; l-His: l-histidine; l-Cys: l-cysteine; LC-ESI-MS: liquid chromatography-electrospray ionization-mass spectrometry.
|
巻・号 |
83(1)
|
ページ |
181-184
|
公開日 |
2019-1-1
|
DOI |
10.1080/09168451.2018.1527210
|
PMID |
30286703
|
MeSH |
Antioxidants / metabolism
Aspergillus oryzae / metabolism*
Chromatography, Liquid
Ergothioneine / biosynthesis*
Ergothioneine / genetics
Fermentation
Genes, Fungal
Neurospora crassa / genetics
Spectrometry, Mass, Electrospray Ionization
|
IF |
1.297
|
引用数 |
4
|
リソース情報 |
一般微生物 |
JCM19069 |