RRC ID |
60365
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著者 |
Nakahara N, Nobu MK, Takaki Y, Miyazaki M, Tasumi E, Sakai S, Ogawara M, Yoshida N, Tamaki H, Yamanaka Y, Katayama A, Yamaguchi T, Takai K, Imachi H.
|
タイトル |
Aggregatilinea lenta gen. nov., sp. nov., a slow-growing, facultatively anaerobic bacterium isolated from subseafloor sediment, and proposal of the new order Aggregatilineales ord. nov. within the class Anaerolineae of the phylum Chloroflexi.
|
ジャーナル |
Int J Syst Evol Microbiol
|
Abstract |
A novel slow-growing, facultatively anaerobic, filamentous bacterium, strain MO-CFX2T, was isolated from a methanogenic microbial community in a continuous-flow bioreactor that was established from subseafloor sediment collected off the Shimokita Peninsula of Japan. Cells were multicellular filamentous, non-motile and Gram-stain-negative. The filaments were generally more than 20 µm (up to approximately 200 µm) long and 0.5-0.6 µm wide. Cells possessed pili-like structures on the cell surface and a multilayer structure in the cytoplasm. Growth of the strain was observed at 20-37 °C (optimum, 30 °C), pH 5.5-8.0 (pH 6.5-7.0), and 0-30 g l-1 NaCl (5 g l-1 NaCl). Under optimum growth conditions, doubling time and maximum cell density were estimated to be approximately 19 days and ~105 cells ml-1, respectively. Strain MO-CFX2T grew chemoorganotrophically on a limited range of organic substrates in anaerobic conditions. The major cellular fatty acids were saturated C16 : 0 (47.9 %) and C18 : 0 (36.9 %), and unsaturated C18 : 1ω9c (6.0 %) and C16 : 1ω7 (5.1 %). The G+C content of genomic DNA was 63.2 mol%. 16S rRNA gene-based phylogenetic analysis showed that strain MO-CFX2T shares a notably low sequence identity with its closest relatives, which were Thermanaerothrix daxensis GNS-1T and Thermomarinilinea lacunifontana SW7T (both 85.8 % sequence identity). Based on these phenotypic and genomic properties, we propose the name Aggregatilinea lenta gen. nov., sp. nov. for strain MO-CFX2T (=KCTC 15625T, =JCM 32065T). In addition, we also propose the associated family and order as Aggregatilineaceae fam. nov. and Aggregatilineales ord. nov., respectively.
|
巻・号 |
69(4)
|
ページ |
1185-1194
|
公開日 |
2019-4-1
|
DOI |
10.1099/ijsem.0.003291
|
PMID |
30775966
|
MeSH |
Bacterial Typing Techniques
Base Composition
Bioreactors / microbiology*
Chloroflexi / classification*
Chloroflexi / isolation & purification
DNA, Bacterial / genetics
Fatty Acids / chemistry
Geologic Sediments / microbiology*
Japan
Phylogeny*
RNA, Ribosomal, 16S / genetics
Seawater / microbiology*
Sequence Analysis, DNA
|
IF |
2.166
|
引用数 |
4
|
リソース情報 |
一般微生物 |
JCM32065 |