RRC ID 60374
著者 Sorokin DY, Khijniak TV, Elcheninov AG, Toshchakov SV, Kostrikina NA, Bale NJ, Sinninghe Damsté JS, Kublanov IV.
タイトル Halococcoides cellulosivorans gen. nov., sp. nov., an extremely halophilic cellulose-utilizing haloarchaeon from hypersaline lakes.
ジャーナル Int J Syst Evol Microbiol
Abstract An extremely halophilic euryarchaeon, strain HArcel1T, was enriched and isolated in pure culture from the surface brines and sediments of hypersaline athalassic lakes in the Kulunda Steppe (Altai region, Russia) using amorphous cellulose as the growth substrate. The colonies of HArcel1T are pale-orange, and form large zones of cellulose hydrolysis around them. The cells are non-motile cocci of variable size with a thin monolayer cell wall. The isolate is an obligate aerobic heterotroph capable of growth with only three substrates: various forms of insoluble cellulose, xylan and cellobiose. Strain HArcel1T is an extremely halophilic neutrophile, growing within the salinity range from 2.5 to 5 M NaCl (optimum at 3.5-4 M). The core archaeal lipids are dominated by C20-C20 and C25-C20 dialkyl glycerol ethers, in approximately 6:1 proportion. The 16S rRNA and rpoB' gene analysis indicated that HArcel1T forms a separate lineage within the family Haloarculaceae, order Halobacteriales, with the genera Halorhabdus and Halopricus as closest relatives. On the basis of the unique phenotypic properties and distinct phylogeny of the 16S rRNA and rpoB' genes, it is suggested that strain HArcel1T is classified into a new genus and species Halococcoides cellulosivorans gen. nov., sp. nov. (JCM 31941T=UNIQEM U975T).
巻・号 69(5)
ページ 1327-1335
公開日 2019-5-1
DOI 10.1099/ijsem.0.003312
PMID 30801242
MeSH Cellulose DNA, Archaeal / genetics Fatty Acids / chemistry Genes, Archaeal Geologic Sediments / microbiology* Halobacteriales / classification* Halobacteriales / isolation & purification Lakes / microbiology* Phylogeny* RNA, Ribosomal, 16S / genetics Russia Salts* Sequence Analysis, DNA
IF 2.166
引用数 2
リソース情報
一般微生物 JCM31941 JCM14471