RRC ID 60655
Author Kuncharoen N, Kudo T, Yuki M, Okuma M, Booncharoen A, Tanasupawat S.
Title Micromonospora musae sp. nov., an endophytic actinomycete isolated from roots of Musa species.
Journal Syst. Appl. Microbiol.
Abstract Two novel actinobacterial strains, MS1-9T and NGC1-4, were isolated from roots of Musa (ABB) cv. 'Kluai Namwa', collected from Chachoengsao province, and Musa (ABB) cv. 'Kluai Chang', from Suphan Buri province, Thailand, respectively. Comparative analysis of 16S rRNA gene (98.0 to 98.9% similarity), gyrase subunit B (gyrB) gene and whole-genome sequences emphasised that the strains MS1-9T and NGC1-4 showed closely related with Micromonospora peucetia DSM 43363T, M. krabiensis JCM 12869T and M. avicenniae DSM 45758T, respectively. Strains MS1-9T and NGC1-4 contained meso-diaminopimelic acid in cell-wall peptidoglycan. Whole-cell sugars were glucose, xylose, mannose, and ribose. The acyl type of peptidoglycan was glycolyl. MK-10(H6), MK-9(H6), and MK-10(H8) were presented as the major menaquinones. Diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, and phosphatidylinositol were detected as predominant phospholipid profiles. The major cellular fatty acids consisted of iso-C15:0, anteiso-C15:0, anteiso-C17:0, iso-C17:0 and C17:0. The DNA G+C content of strains MS1-9T and NGC1-4 were 72.2 and 72.3mol%, respectively. Draft genome sequences indicated by ANI values and digital DNA-DNA hybridisation analysis asserted that the strains MS1-9T and NGC1-4 should be represented as a novel species within the genus Micromonospora for which the name Micromonospora musae sp. nov. is proposed. The type strain is MS1-9T (=JCM 32149T=TISTR 2659T).
Volume 42(6)
Pages 126020
Published 2019-11
DOI 10.1016/j.syapm.2019.126020
PII S0723-2020(19)30315-7
PMID 31601450
MeSH Base Composition Cell Wall / chemistry DNA, Bacterial / genetics Diaminopimelic Acid / analysis Fatty Acids / analysis Genome, Bacterial / genetics Micromonospora / chemistry Micromonospora / classification* Micromonospora / genetics Micromonospora / ultrastructure Musa / microbiology* Nucleic Acid Hybridization Peptidoglycan / analysis Phospholipids / analysis Phylogeny* Plant Roots / microbiology* RNA, Ribosomal, 16S / genetics Sequence Analysis, DNA Species Specificity Sugars / analysis Thailand Vitamin K 2 / analysis
IF 2.808
Times Cited 0
Resource
General Microbes JCM32149 JCM12869 JCM12677 JCM9459 JCM12820 JCM31034