RRC ID 60684
Author Gimenez-Ibanez S, Boter M, Ortigosa A, García-Casado G, Chini A, Lewsey MG, Ecker JR, Ntoukakis V, Solano R.
Title JAZ2 controls stomata dynamics during bacterial invasion.
Journal New Phytol
Abstract Coronatine (COR) facilitates entry of bacteria into the plant apoplast by stimulating stomata opening. COR-induced signaling events at stomata remain unclear. We found that the COR and jasmonate isoleucine (JA-Ile) co-receptor JAZ2 is constitutively expressed in guard cells and modulates stomatal dynamics during bacterial invasion We analyzed tissue expression patterns of AtJAZ genes and measured stomata opening and pathogen resistance in loss- and gain-of-function mutants. Arabidopsis jaz2 mutants are partially impaired in pathogen-induced stomatal closing and more susceptible to Pseudomonas. Gain-of-function mutations in JAZ2 prevent stomatal reopening by COR and are highly resistant to bacterial penetration. The JAZ2 targets MYC2, MYC3 and MYC4 directly regulate the expression of ANAC19, ANAC55 and ANAC72 to modulate stomata aperture. Due to the antagonistic interactions between the salicylic acid (SA) and JA defense pathways, efforts to increase resistance to biotrophs result in enhanced susceptibility to necrotrophs, and vice versa. Remarkably, dominant jaz2Δjas mutants are resistant to Pseudomonas syringae but retain unaltered resistance against necrotrophs. Our results demonstrate the existence of a COI1-JAZ2-MYC2,3,4-ANAC19,55,72 module responsible for the regulation of stomatal aperture that is hijacked by bacterial COR to promote infection. They also provide novel strategies for crop protection against biotrophs without compromising resistance to necrotrophs.
Volume 213(3)
Pages 1378-1392
Published 2017-2-1
DOI 10.1111/nph.14354
PMID 28005270
MeSH Amino Acids / pharmacology Arabidopsis / drug effects Arabidopsis / genetics Arabidopsis / microbiology* Arabidopsis Proteins / genetics Arabidopsis Proteins / metabolism* Disease Resistance / drug effects Gene Expression Regulation, Plant / drug effects Genes, Dominant Indenes / pharmacology Mutation / genetics Organ Specificity / genetics Plant Diseases / microbiology Plant Stomata / cytology Plant Stomata / drug effects Plant Stomata / microbiology* Plant Stomata / physiology Pseudomonas syringae / drug effects Pseudomonas syringae / pathogenicity Repressor Proteins / genetics Repressor Proteins / metabolism*
IF 7.299
Times Cited 49
Arabidopsis / Cultured plant cells, genes pst17092