論文 - 詳細
| RRC ID | 60783 |
|---|---|
| 著者 | Lee SR, Hong ST, Choi KW. |
| タイトル | Regulation of epithelial integrity and organ growth by Tctp and Coracle in Drosophila. |
| ジャーナル | PLoS Genet |
| Abstract |
Regulation of cell junctions is crucial for the integrity of epithelial tissues and organs. Cell junctions also play roles in controlling cell proliferation for organ growth. Translationally controlled tumor protein (TCTP) is a conserved protein involved in growth control, but its role in cell junctions is unknown. Here we show that Drosophila Tctp directly interacts with the septate junction protein Coracle (Cora) to regulate epithelial integrity and organ growth. Tctp localizes together with Cora in the epidermis of the embryo. Loss of Cora reduces the level of Tctp in the epidermis but not vice versa. cora/+ or Tctp/+ single heterozygotes develop normally to adulthood. However, double heterozygotes for cora and Tctp mutations show severe disruption of epithelia causing synthetic lethality in the embryo. Double knockdown of Cora and Tctp in eye imaginal disc synergistically leads to disruption of the eye disc, resulting in a severe reduction or loss of eye and head. Conversely, double knockdown of Cora and Tctp in wing disc causes overgrowth as well as cell death. Inhibition of cell death under this condition causes hyperplastic growth of the wing disc. Tctp also shows direct and functional interaction with Cora-associated factors like Yurt and Na+/K+-ATPase. This study suggests that proper levels of Tctp and Cora are essential for the maintenance of the Cora complex and the integrity of epithelia. Our data also provide evidence that both Cora and Tctp are required to suppress overgrowth in developing wing. |
| 巻・号 | 16(6) |
| ページ | e1008885 |
| 公開日 | 2020-6-1 |
| DOI | 10.1371/journal.pgen.1008885 |
| PII | PGENETICS-D-19-00997 |
| PMID | 32559217 |
| PMC | PMC7329144 |
| MeSH | Animals Animals, Genetically Modified Cell Proliferation / genetics Drosophila Proteins / genetics Drosophila Proteins / metabolism* Drosophila melanogaster / physiology* Embryo, Nonmammalian Epithelium / metabolism* Female Gene Expression Regulation, Developmental Gene Knockdown Techniques Imaginal Discs / growth & development Intercellular Junctions / metabolism Intracellular Signaling Peptides and Proteins / genetics Intracellular Signaling Peptides and Proteins / metabolism* Male Membrane Proteins / genetics Membrane Proteins / metabolism* Morphogenesis / genetics Synthetic Lethal Mutations Wings, Animal / growth & development* Wings, Animal / metabolism |
| IF | 5.175 |
| 引用数 | 0 |
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 3 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
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