RRC ID 60874
Author Higuchi Y, Hisamatsu T.
Title CsTFL1, a constitutive local repressor of flowering, modulates floral initiation by antagonising florigen complex activity in chrysanthemum.
Journal Plant Sci
Abstract Chrysanthemums require repeated cycles of short-day (SD) photoperiod for successful anthesis, but their vegetative state is strictly maintained under long-day (LD) or night-break (NB) conditions. We have previously demonstrated that photoperiodic flowering of a wild diploid chrysanthemum (Chrysanthemum seticuspe f. boreale) is controlled by a pair of systemic floral regulators, florigen (CsFTL3) and anti-florigen (CsAFT), produced in the leaves. Here, we report the functional characterisation of a local floral regulator, CsTFL1, a chrysanthemum orthologue of TERMINAL FLOWER 1 gene in Arabidopsis. Constitutive expression of CsTFL1 in C. seticuspe (CsTFL1-ox) resulted in extremely late flowering under SD and prevented up-regulation of floral meristem identity genes in shoot tips and leaves. Bimolecular fluorescence complementation assay showed that both CsTFL1 and CsFTL3 interacted with CsFDL1, a bZIP transcription factor FD homologue, in the nucleus. The transient gene expression assay indicated that CsTFL1 suppresses flowering by directly antagonising the flower inductive activity of the CsFTL3-CsFDL1 complex. Our results suggest that strict maintenance of vegetative state under non-inductive photoperiod is achieved by the coordinated action of both the systemic floral inhibitor and local floral inhibitor CsTFL1, which is constitutively expressed in shoot tips.
Volume 237
Pages 1-7
Published 2015-8-1
DOI 10.1016/j.plantsci.2015.04.011
PII S0168-9452(15)00111-9
PMID 26089146
MeSH Chrysanthemum / genetics* Chrysanthemum / growth & development Chrysanthemum / radiation effects Florigen / antagonists & inhibitors* Flowers / genetics* Flowers / growth & development Flowers / radiation effects Gene Expression Regulation, Developmental Gene Expression Regulation, Plant* Light Meristem / genetics Meristem / growth & development Meristem / radiation effects Photoperiod Plant Growth Regulators / antagonists & inhibitors* Plant Leaves / genetics Plant Leaves / radiation effects Plant Proteins / genetics Plant Proteins / metabolism Plant Shoots / genetics Plant Shoots / growth & development Plant Shoots / radiation effects Plants, Genetically Modified Repressor Proteins / genetics* Repressor Proteins / metabolism Up-Regulation
IF 3.785
Times Cited 12
Chrysanthemum NIFS-3