論文 - 詳細
| RRC ID | 60892 |
|---|---|
| 著者 | Aso H, Nagaoka S, Kawakami E, Ito J, Islam S, Tan BJY, Nakaoka S, Ashizaki K, Shiroguchi K, Suzuki Y, Satou Y, Koyanagi Y, Sato K. |
| タイトル | Multiomics Investigation Revealing the Characteristics of HIV-1-Infected Cells In Vivo. |
| ジャーナル | Cell Rep |
| Abstract |
For eradication of HIV-1 infection, it is important to elucidate the detailed features and heterogeneity of HIV-1-infected cells in vivo. To reveal multiple characteristics of HIV-1-producing cells in vivo, we use a hematopoietic-stem-cell-transplanted humanized mouse model infected with GFP-encoding replication-competent HIV-1. We perform multiomics experiments using recently developed technology to identify the features of HIV-1-infected cells. Genome-wide HIV-1 integration-site analysis reveals that productive HIV-1 infection tends to occur in cells with viral integration into transcriptionally active genomic regions. Bulk transcriptome analysis reveals that a high level of viral mRNA is transcribed in HIV-1-infected cells. Moreover, single-cell transcriptome analysis shows the heterogeneity of HIV-1-infected cells, including CXCL13high cells and a subpopulation with low expression of interferon-stimulated genes, which can contribute to efficient viral spread in vivo. Our findings describe multiple characteristics of HIV-1-producing cells in vivo, which could provide clues for the development of an HIV-1 cure. |
| 巻・号 | 32(2) |
| ページ | 107887 |
| 公開日 | 2020-7-14 |
| DOI | 10.1016/j.celrep.2020.107887 |
| PII | S2211-1247(20)30868-8 |
| PMID | 32668246 |
| MeSH | Animals Female Genomics* Green Fluorescent Proteins / metabolism HEK293 Cells HIV Infections / genetics* HIV Infections / metabolism* HIV-1 / physiology* Humans Male Mice Transcriptome / genetics |
| IF | 8.109 |
| 引用数 | 0 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 39 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 遺伝子材料 | CSII-CMV-MCS-IRES2-Bsd (RDB04385) pCMV-VSV-G-RSV-Rev (RDB04393) pCAG-HIVgp (RDB04394) |