RRC ID 6105
著者 Lu Q, Tang X, Tian G, Wang F, Liu K, Nguyen V, Kohalmi SE, Keller WA, Tsang EW, Harada JJ, Rothstein SJ, Cui Y.
タイトル Arabidopsis homolog of the yeast TREX-2 mRNA export complex: components and anchoring nucleoporin.
ジャーナル Plant J
Abstract Nuclear pore complexes (NPCs) are vital to nuclear-cytoplasmic communication in eukaryotes. The yeast NPC-associated TREX-2 complex, also known as the Thp1-Sac3-Cdc31-Sus1 complex, is anchored on the NPC via the nucleoporin Nup1, and is essential for mRNA export. Here we report the identification and characterization of the putative Arabidopsis thaliana TREX-2 complex and its anchoring nucleoporin. Physical and functional evidence support the identification of the Arabidopsis orthologs of yeast Thp1 and Nup1. Of three Arabidopsis homologs of yeast Sac3, two are putative TREX-2 components, but, surprisingly, none are required for mRNA export as they are in yeast. Physical association of the two Cdc31 homologs, but not the Sus1 homolog, with the TREX-2 complex was observed. In addition to identification of these TREX-2 components, direct interactions of the Arabidopsis homolog of DSS1, which is an established proteasome component in yeast and animals, with both the TREX-2 complex and the proteasome were observed. This suggests the possibility of a link between the two complexes. Thus this work has identified the putative Arabidopsis TREX-2 complex and provides a foundation for future studies of nuclear export in Arabidopsis.
巻・号 61(2)
ページ 259-70
公開日 2010-1-1
DOI 10.1111/j.1365-313X.2009.04048.x
PII TPJ4048
PMID 19843313
MeSH Active Transport, Cell Nucleus Arabidopsis / genetics Arabidopsis / metabolism* Arabidopsis Proteins / classification Arabidopsis Proteins / genetics Arabidopsis Proteins / metabolism* Cell Nucleus / metabolism Gene Expression Regulation, Plant Luminescent Proteins / genetics Luminescent Proteins / metabolism Models, Biological Mutation Nuclear Pore / metabolism* Nuclear Pore Complex Proteins / genetics Nuclear Pore Complex Proteins / metabolism* Phylogeny Plants, Genetically Modified Proteasome Endopeptidase Complex / metabolism Protein Binding RNA Transport RNA, Messenger / genetics RNA, Messenger / metabolism Reverse Transcriptase Polymerase Chain Reaction Saccharomyces cerevisiae / genetics Saccharomyces cerevisiae / metabolism Saccharomyces cerevisiae Proteins / genetics Saccharomyces cerevisiae Proteins / metabolism Two-Hybrid System Techniques
IF 6.141
引用数 177
WOS 分野 PLANT SCIENCES
リソース情報
シロイヌナズナ / 植物培養細胞・遺伝子 pda11926