RRC ID 61098
Author Katsuyama Y, Taira N, Yoshioka M, Okano Y, Masaki H.
Title Disruption of melanosome transport in melanocytes treated with theophylline causes their degradation by autophagy.
Journal Biochem Biophys Res Commun
Abstract Melanosomes containing melanin are transported from the perinuclear area to the tips of dendrites in epidermal melanocytes, and are then transferred to keratinocytes. Thus, skin color is determined by the amount of melanin synthesized in melanocytes and the subsequent dispersion of melanosomes in the epidermis. Therefore, disrupting intracellular melanosome transport in melanocytes is considered an effective approach to regulate skin color. However, the fate of melanosomes that accumulate in melanocytes due to disrupted intracellular transport is unclear. In this study, we disrupted melanosome transport by knockdown of the motor protein MyosinVa. Knock-down of MyosinVa (M-KD) in cells treated with theophylline significantly down-regulated the mRNA and protein expression levels of tyrosinase. Interestingly, intracellular melanin contents in M-KD cells were decreased. Furthermore, M-KD cells showed activation of autophagy through increased expression of Microtubule-associated protein 1 light chain 3 (LC3) -II and decreased expression of p62. The sum of these results indicate that disruption of melanosome transport causes their degradation by autophagy.
Volume 485(1)
Pages 126-130
Published 2017-3-25
DOI 10.1016/j.bbrc.2017.02.033
PII S0006-291X(17)30301-7
PMID 28188783
MeSH Animals Autophagy / drug effects* Biological Transport / drug effects Cell Line, Tumor Gene Expression Regulation / drug effects Gene Knockdown Techniques Melanins / genetics Melanins / metabolism Melanocytes / cytology Melanocytes / drug effects* Melanocytes / metabolism Melanoma / genetics Melanoma / metabolism Melanosomes / drug effects* Melanosomes / metabolism Mice Microtubule-Associated Proteins / genetics Microtubule-Associated Proteins / metabolism Monophenol Monooxygenase / genetics Monophenol Monooxygenase / metabolism Myosin Heavy Chains / genetics* Myosin Heavy Chains / metabolism* Myosin Type V / genetics* Myosin Type V / metabolism* RNA Interference RNA, Small Interfering / genetics Theophylline / pharmacology*
IF 2.985
Human and Animal Cells B16 mouse melanoma