RRC ID 61105
Author Hiruta Y, Nemoto R, Kanazawa H.
Title Design and synthesis of temperature-responsive polymer/silica hybrid nanoparticles and application to thermally controlled cellular uptake.
Journal Colloids Surf B Biointerfaces
Abstract This study reports the development of temperature-responsive polymer/silica hybrid nanoparticles and their application to temperature-dependent intracellular uptake of hydrophobic encapsulated fluorescence molecules. Amphiphilic diblock copolymer comprising a temperature-responsive segment, poly(N-isopropylacrylamide-co-N,N-dimethylacrylamide) [P(NIPAAm-co-DMAAm)] and a trimethyoxysilyl-containing hydrophobic segment was synthesized (PBM-b-ND); this amphiphilic diblock copolymer self-assembled in an aqueous solution, and temperature-responsive polymer/silica hybrid fluorescence nanoparticles were fabricated via a base-catalyzed sol-gel process. The fluorescence probe rhodamine DHPE or boron dipyrromethene derivative was encapsulated into the polymer core with a silica network in a stable manner. Other types of polymer/silica hybrid fluorescence nanoparticles were also developed using either homo-PNIPAAm (PBM-b-N) or homo-PDMAAm (PBM-b-D) segments, instead of P(NIPAAm-co-DMAAm). While PBM-b-D did not exhibit a temperature-dependent phase transition (hydrophilic characteristic), PBM-b-N and PBM-b-ND exhibited temperature-dependent phase transition (hydrophilic/hydrophobic) at 32°C and 38°C, respectively. The cellular uptake of PBM-b-N was clearly observed at both 37°C and 42°C, while the cellular uptake of PBM-b-D was minimal at these temperatures. On the other hand, significant enhancement in the intracellular uptake of PBM-b-ND was observed at 42°C, compared to its uptake at a lower temperature of 37°C. These results indicated that temperature-responsive polymer/silica hybrid nanoparticle, PBM-b-ND demonstrate potential for applications in theranostics with cancer therapy via the combination of local drug delivery and local hyperthermia, as well as for monitoring treatment effectiveness with fluorescence imaging.
Volume 153
Pages 2-9
Published 2017-5-1
DOI 10.1016/j.colsurfb.2017.01.052
PII S0927-7765(17)30071-1
PMID 28192714
MeSH Drug Design* Flow Cytometry Fluorescent Dyes / chemical synthesis Fluorescent Dyes / chemistry Fluorescent Dyes / pharmacokinetics HeLa Cells Humans Hydrophobic and Hydrophilic Interactions Microscopy, Fluorescence Molecular Structure Nanoparticles / chemistry* Polymers / chemical synthesis* Polymers / chemistry Polymers / pharmacokinetics* Silicon Dioxide / chemistry Silicon Dioxide / pharmacokinetics* Surface-Active Agents / chemical synthesis Surface-Active Agents / chemistry Surface-Active Agents / pharmacokinetics Temperature* Tumor Cells, Cultured
IF 4.389
Human and Animal Cells HeLa(RCB0007)