論文 - 詳細
| RRC ID | 61105 |
|---|---|
| 著者 | Hiruta Y, Nemoto R, Kanazawa H. |
| タイトル | Design and synthesis of temperature-responsive polymer/silica hybrid nanoparticles and application to thermally controlled cellular uptake. |
| ジャーナル | Colloids Surf B Biointerfaces |
| Abstract |
This study reports the development of temperature-responsive polymer/silica hybrid nanoparticles and their application to temperature-dependent intracellular uptake of hydrophobic encapsulated fluorescence molecules. Amphiphilic diblock copolymer comprising a temperature-responsive segment, poly(N-isopropylacrylamide-co-N,N-dimethylacrylamide) [P(NIPAAm-co-DMAAm)] and a trimethyoxysilyl-containing hydrophobic segment was synthesized (PBM-b-ND); this amphiphilic diblock copolymer self-assembled in an aqueous solution, and temperature-responsive polymer/silica hybrid fluorescence nanoparticles were fabricated via a base-catalyzed sol-gel process. The fluorescence probe rhodamine DHPE or boron dipyrromethene derivative was encapsulated into the polymer core with a silica network in a stable manner. Other types of polymer/silica hybrid fluorescence nanoparticles were also developed using either homo-PNIPAAm (PBM-b-N) or homo-PDMAAm (PBM-b-D) segments, instead of P(NIPAAm-co-DMAAm). While PBM-b-D did not exhibit a temperature-dependent phase transition (hydrophilic characteristic), PBM-b-N and PBM-b-ND exhibited temperature-dependent phase transition (hydrophilic/hydrophobic) at 32°C and 38°C, respectively. The cellular uptake of PBM-b-N was clearly observed at both 37°C and 42°C, while the cellular uptake of PBM-b-D was minimal at these temperatures. On the other hand, significant enhancement in the intracellular uptake of PBM-b-ND was observed at 42°C, compared to its uptake at a lower temperature of 37°C. These results indicated that temperature-responsive polymer/silica hybrid nanoparticle, PBM-b-ND demonstrate potential for applications in theranostics with cancer therapy via the combination of local drug delivery and local hyperthermia, as well as for monitoring treatment effectiveness with fluorescence imaging. |
| 巻・号 | 153 |
| ページ | 2-9 |
| 公開日 | 2017-5-1 |
| DOI | 10.1016/j.colsurfb.2017.01.052 |
| PII | S0927-7765(17)30071-1 |
| PMID | 28192714 |
| MeSH | Drug Design* Flow Cytometry Fluorescent Dyes / chemical synthesis Fluorescent Dyes / chemistry Fluorescent Dyes / pharmacokinetics HeLa Cells Humans Hydrophobic and Hydrophilic Interactions Microscopy, Fluorescence Molecular Structure Nanoparticles / chemistry* Polymers / chemical synthesis* Polymers / chemistry Polymers / pharmacokinetics* Silicon Dioxide / chemistry Silicon Dioxide / pharmacokinetics* Surface-Active Agents / chemical synthesis Surface-Active Agents / chemistry Surface-Active Agents / pharmacokinetics Temperature* Tumor Cells, Cultured |
| IF | 4.389 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 各媒体での言及数の合計 | 0 |
| リソース情報 | |
| ヒト・動物細胞 | HeLa(RCB0007) |