論文 - 詳細
| RRC ID | 61128 |
|---|---|
| 著者 | Nishimiya O, Katsu Y, Inagawa H, Hiramatsu N, Todo T, Hara A. |
| タイトル | Molecular cloning and characterization of hagfish estrogen receptors. |
| ジャーナル | J Steroid Biochem Mol Biol |
| Abstract |
One or more distinct forms of the nuclear estrogen receptor (ER) have been isolated from many vertebrates to date. To better understand the molecular evolution of ERs, we cloned and characterized er cDNAs from the inshore hagfish, Eptatretus burgeri, a modern representative of the most primitive vertebrates, the agnathans. Two er cDNAs, er1 and er2, were isolated from the liver of a reproductive female hagfish. A phylogenetic analysis placed hagfish ER1 into a position prior to the divergence of vertebrate ERs. Conversely, hagfish ER2 was placed at the base of the vertebrate ERβ clade. The tissue distribution patterns of both ER subtype mRNAs appeared to be different, suggesting that each subtype has different physiological roles associated with estrogen actions. An estrogen responsive-luciferase reporter assay using mammalian HEK293 cells was used to functionally characterize these hagfish ERs. Both ER proteins displayed estrogen-dependent activation of transcription. These results clearly demonstrate that the hagfish has two functional ER subtypes. |
| 巻・号 | 165(Pt B) |
| ページ | 190-201 |
| 公開日 | 2017-1-1 |
| DOI | 10.1016/j.jsbmb.2016.06.004 |
| PII | S0960-0760(16)30174-1 |
| PMID | 27287934 |
| MeSH | Animals Cloning, Molecular DNA, Complementary / metabolism Dose-Response Relationship, Drug Estrogen Receptor alpha / genetics Estrogen Receptor beta / genetics Estrogens / metabolism* Evolution, Molecular Female Fish Proteins / genetics Fish Proteins / metabolism* HEK293 Cells Hagfishes / genetics* Hagfishes / metabolism Humans Ligands Male Phylogeny Receptors, Estrogen / genetics Receptors, Estrogen / metabolism* Signal Transduction Species Specificity Tissue Distribution Transcriptional Activation |
| IF | 3.813 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 各媒体での言及数の合計 | 0 |
| リソース情報 | |
| ヒト・動物細胞 | 293(RCB1637) |