RRC ID 61171
Author Itaya M, Sato M, Watanabe S, Yoshikawa H, Tomita M, Sato R.
Title Stable mutants of restriction-deficient/modification-proficient Bacillus subtilis 168: hub strains for giant DNA engineering.
Journal J Biochem
Abstract Bacillus subtilis 168 has been explored as a platform for the synthesis and transmission of large DNA. Two inherent DNA incorporation systems, natural transformation and pLS20-based conjugation transfer, enable rapid handling of target DNA. Both systems are affected by the Bsu restriction-modification system that recognizes and cleaves unmethylated XhoI sites, limiting the choice of target DNA. We constructed B. subtilis 168 with stable mutation for restriction-deficient and modification-proficient (r-m+). It was demonstrated that the r-m+ strains can incorporate and transfer synthesized DNA with multiple XhoI sites. These should be of value as hub strains to integrate and disseminate giant DNA between B. subtilis 168 derivatives.
Volume 166(3)
Pages 231-236
Published 2019-9-1
DOI 10.1093/jb/mvz031
PII 5475605
PMID 31004491
IF 2.23
Resource
Prokaryotes B. subtilis MBS829 MBS830 MBS831 MBS832 MBS833 MBS834 MBS835 MBS836 MBS837 MBS838 MBS839