RRC ID 6119
著者 Fujii T, Kuwazaki S, Yamamoto K, Abe H, Ohnuma A, Katsuma S, Mita K, Shimada T.
タイトル Identification and molecular characterization of a sex chromosome rearrangement causing a soft and pliable (spli) larval body phenotype in the silkworm, Bombyx mori.
ジャーナル Genome
Abstract We carried out genetic and cytogenetic analyses of X-ray-induced deleterious Z chromosomes that result in a soft and pliable (spli) phenotype in the silkworm, Bombyx mori. In a B. mori strain with a spli phenotype, we found the Z chromosome broken between the sch (1-21.5) and od (1-49.6) loci. We also found a chromosomal fragment bearing a fifth-chromosome locus for egg and eye pigmentation fused to a Z chromosome fragment. By means of fluorescence in situ hybridization using bacterial artificial chromosome clones as probes, we confirmed that the fused chromosome is composed of a fragment of chromosome 5 and a fragment of the Z chromosome. Moreover, a predicted gene, GA002017, the Bombyx ortholog of the Drosophila gene acj6 (Bmacj6), was completely deleted by the Z chromosome breakage event. The relationship between Bmacj6 and the spli phenotype is discussed.
巻・号 53(1)
ページ 45-54
公開日 2010-1-1
DOI 10.1139/g09-083
PII g09-083
解説 spli(エスプリ)の原因遺伝子はBmacj6である。Bmacj6はPOUドメインを持った転写因子をコードする。n41系統(spli/spli)のZ染色体は二つに切断されており、切断によって、Bmacj6のエクソン2~4が欠失している。
PMID 20130748
MeSH Animals Bombyx / anatomy & histology* Bombyx / genetics* Bombyx / growth & development Chromosome Breakage Chromosome Mapping Cloning, Molecular Female Genetic Loci Genetic Markers / physiology Larva / anatomy & histology* Larva / genetics* Male Models, Biological Phenotype Sequence Deletion Sex Chromosome Aberrations* Sex Chromosomes / genetics Somatotypes / genetics
IF 2.037
引用数 4
WOS 分野 BIOTECHNOLOGY & APPLIED MICROBIOLOGY GENETICS & HEREDITY
リソース情報
カイコ p50 n41 u01