Reference - Detail
| RRC ID | 61191 |
|---|---|
| Author | Tateno H, Onuma Y, Ito Y, Hiemori K, Aiki Y, Shimizu M, Higuchi K, Fukuda M, Warashina M, Honda S, Asashima M, Hirabayashi J. |
| Title | A medium hyperglycosylated podocalyxin enables noninvasive and quantitative detection of tumorigenic human pluripotent stem cells. |
| Journal | Sci Rep |
| Abstract |
While human pluripotent stem cells are attractive sources for cell-replacement therapies, a major concern remains regarding their tumorigenic potential. Thus, safety assessment of human pluripotent stem cell-based products in terms of tumorigenicity is critical. Previously we have identified a pluripotent stem cell-specific lectin probe rBC2LCN recognizing hyperglycosylated podocalyxin as a cell surface ligand. Here we demonstrate that hyperglycosylated podocalyxin is secreted from human pluripotent stem cells into cell culture supernatants. We establish a sandwich assay system, named the GlycoStem test, targeting the soluble hyperglycosylated podocalyxin using rBC2LCN. The GlycoStem test is sufficiently sensitive and quantitative to detect residual human pluripotent stem cells. This work provides a proof of concept for the noninvasive and quantitative detection of tumorigenic human pluripotent stem cells using cell culture supernatants. The developed method should increase the safety of human pluripotent stem cell-based cell therapies. |
| Volume | 4 |
| Pages | 4069 |
| Published | 2014-2-12 |
| DOI | 10.1038/srep04069 |
| PII | srep04069 |
| PMID | 24518842 |
| PMC | PMC3921628 |
| MeSH | Biotin / chemistry Biotinylation Burkholderia cenocepacia / metabolism Cell Differentiation Cell Transformation, Neoplastic* Cells, Cultured Coculture Techniques Flow Cytometry Glycosylation HEK293 Cells Humans Lectins / chemistry Lectins / genetics Lectins / metabolism Ligands Pluripotent Stem Cells / cytology* Pluripotent Stem Cells / metabolism Protein Array Analysis / methods Sialoglycoproteins / chemistry Sialoglycoproteins / metabolism* |
| IF | 3.998 |
| Resource | |
| Human and Animal Cells | 201B7(HPS0063) 253G1(HPS0002) |