RRC ID 61204
Author Inoue H, Ohnishi Y, Nakajima M, Kakudo K, Nozaki M.
Title A novel function of EpCAM in oral squamous cell carcinoma cells under anchorage-independent conditions.
Journal Int J Oncol
Abstract Epithelial cell adhesion molecule (EpCAM), involved in Ca2+-independent homotypic cell-cell adhesion in epithelial tissues, is overexpressed in several cancer types. Although studies investigating the function of EpCAM in cancers have shown that it plays a role in cell proliferation, invasion and metastasis, the overall function of EpCAM in cancer cells has remained elusive. Here, we report a novel function of EpCAM in multicellular aggregates (MCAs). EpCAM inhibition using RNA interference (RNAi) did not affect cell morphology, proliferation or expression of certain genes, including cyclin D1 in monolayer cultures of the human oral squamous cell carcinoma cell lines HSC-3 or HSC-4. However, in HSC-4 cells cultured as MCAs, suppression of EpCAM significantly reduced the expression levels of cyclin D1. Nuclear localization of the cyclin D1 protein was observed in MCAs of HSC-4 cells but not in MCAs of EpCAM knockdown HSC4 cells, suggesting that EpCAM regulates cyclin D1 expression and localization in HSC-4 cells under anchorage-independent conditions. We propose that targeting EpCAM might result in more efficient therapies under certain conditions of oral squamous cell carcinoma.
Volume 39(6)
Pages 1401-5
Published 2011-12-1
DOI 10.3892/ijo.2011.1137
PMID 21785818
MeSH Animals Antigens, Neoplasm / genetics Antigens, Neoplasm / metabolism* Carcinoma, Squamous Cell / genetics Carcinoma, Squamous Cell / metabolism* Cell Adhesion Molecules / genetics Cell Adhesion Molecules / metabolism* Cell Line, Tumor Cell Transformation, Neoplastic / genetics Cell Transformation, Neoplastic / metabolism Cyclin D1 / genetics Cyclin D1 / metabolism Down-Regulation / genetics Epithelial Cell Adhesion Molecule Female Gene Expression Regulation, Neoplastic Humans Male Mice Mouth Neoplasms / genetics Mouth Neoplasms / metabolism* Tongue / metabolism Tongue / pathology
IF 3.571
Human and Animal Cells SAS(RCB1974) HSC-3(RCB1975) HSC-4(RCB1902)