論文 - 詳細
| RRC ID | 61223 |
|---|---|
| 著者 | Miyoshi S, Motohashi T, Nakamura Y, Osawa M, Hiroyama T, Kim DK, Tokumoto Y, Nakauchi H. |
| タイトル | A transmembrane trap method for efficient cloning of genes encoding proteins possessing transmembrane domain. |
| ジャーナル | Biochem Biophys Res Commun |
| Abstract |
To facilitate searching for genes encoding cell membrane proteins, we developed a method for isolating cDNAs that contain sequences for hydrophobic transmembrane runs. This cloning strategy, termed the "transmembrane (TM) trap method," utilizes a vector that directs the cell surface expression of mouse CD4 fusion protein when an insert encoding hydrophobic transmembrane sequences is cloned in-frame with correct orientation. We applied this novel method to isolation of cytokine receptor cDNAs. Our strategy enabled efficient isolation of relatively rare species encoding receptors such as IL-2Rgamma, IL-3Rbeta, IL-4Ralpha, IL-5Ralpha, and IL-6Ralpha. This method also could be used to isolate cDNAs for intracellular molecules with a transmembrane region, e.g., bcl-2. These results indicate that the TM trap method provides an efficient cloning strategy for identification of various families of genes encoding proteins with one or more transmembrane regions. |
| 巻・号 | 289(5) |
| ページ | 1192-8 |
| 公開日 | 2001-12-21 |
| DOI | 10.1006/bbrc.2001.6097 |
| PII | S0006-291X(01)96097-8 |
| PMID | 11741319 |
| MeSH | Animals Base Sequence CD4 Antigens / genetics COS Cells Cell Line Cell Membrane / chemistry Cloning, Molecular / methods* DNA, Complementary / genetics DNA, Complementary / isolation & purification Gene Expression Gene Library Genetic Vectors Humans Membrane Proteins / chemistry* Membrane Proteins / genetics* Mice Plasmids / genetics Protein Structure, Tertiary Recombinant Fusion Proteins / genetics Transfection |
| IF | 2.985 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 2 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | F-36P(RCB0775) |