RRC ID 61224
Author Jawhari AU, Noda M, Farthing MJ, Pignatelli M.
Title Abnormal expression and function of the E-cadherin-catenin complex in gastric carcinoma cell lines.
Journal Br J Cancer
Abstract Dysfunction of the cadherin-catenin complex, a key component of adherens junctions, is thought to confer invasive potential to cells. The aim of this study is to examine the expression and function of the E-cadherin/catenin complex in gastric carcinoma cell lines. Expression of E-cadherin, alpha, beta and gamma-catenin and p120ctn, and of the adenomatous polyposis coli protein (APC), together with function of the cadherin-catenin complex was examined in a panel of gastric carcinoma cell lines, using immunocytochemistry, Western blotting and a cell-cell aggregation assay. Protein interactions were examined by sequential immunoprecipitation and immunoblotting with antibodies to E-cadherin, alpha, beta and gamma-catenin, p120ctn and APC. Abnormalities of E-cadherin, alpha- and beta-catenin expression, were associated with disturbance of E-cadherin-catenin complex composition, loss of membranous localization and loss of calcium-dependent aggregation in six gastric carcinoma cell lines. APC protein expression and interaction with beta-catenin was preserved in five cell lines. We demonstrate frequent abnormalities of expression and function of E-cadherin and catenins, and associated disturbance of E-cadherin-mediated intercellular adhesion in gastric carcinoma cell lines. These findings support the tumour suppressor role of the E-cadherin and its contribution to the development and progression of the neoplastic phenotype in gastric carcinoma.
Volume 80(3-4)
Pages 322-30
Published 1999-5-1
DOI 10.1038/sj.bjc.6690358
PII 6690358
PMID 10408833
PMC PMC2362351
MeSH Animals Cadherins / biosynthesis* Cadherins / metabolism Cadherins / physiology Carcinoma / metabolism* Carcinoma / pathology Cell Adhesion / physiology Cell Aggregation / physiology Cell Communication / physiology Cytoskeletal Proteins / biosynthesis* Cytoskeletal Proteins / metabolism Cytoskeletal Proteins / physiology Humans Macromolecular Substances Mice Mice, Inbred BALB C Mice, Nude Stomach Neoplasms / metabolism* Stomach Neoplasms / pathology Trans-Activators* Tumor Cells, Cultured alpha Catenin beta Catenin
IF 5.791
Human and Animal Cells MKN7(RCB0999) MKN74(RCB1002)