| RRC ID |
61300
|
| 著者 |
Hida K, Shindoh M, Yasuda M, Hanzawa M, Funaoka K, Kohgo T, Amemiya A, Totsuka Y, Yoshida K, Fujinaga K.
|
| タイトル |
Antisense E1AF transfection restrains oral cancer invasion by reducing matrix metalloproteinase activities.
|
| ジャーナル |
Am J Pathol
|
| Abstract |
E1AF is a newly identified human ets-family transcription factor. We have reported that E1AF can up-regulate transcription of matrix metalloproteinase (MMP) genes and confers invasive phenotype on human cancer cells. HSC3 is an oral squamous-cell-carcinoma-derived cell line, and it manifests high levels of E1AF and MMP-1 and -9 gene expression that are associated with invasive potential. We reconstructed an E1AF antisense expression vector, transfected HSC3 cells with the vector, and obtained HSC3AS cells that express E1AF antisense RNA. HSC3AS showed decreasing mRNA and protein levels of MMP-1, -3, and -9. Moreover, HSC3AS showed lower invasive potential in vitro three-dimensional raft culture and in vivo implantation into nude mice. These results imply that transfection of antisense E1AF inhibits tumor invasion by down-regulating MMP genes.
|
| 巻・号 |
150(6)
|
| ページ |
2125-32
|
| 公開日 |
1997-6-1
|
| PMID |
9176403
|
| PMC |
PMC1858336
|
| MeSH |
Adenovirus E1A Proteins / genetics*
Animals
Blotting, Northern
Carcinoma, Squamous Cell / enzymology*
Carcinoma, Squamous Cell / genetics*
Carcinoma, Squamous Cell / pathology
Cell Culture Techniques / methods
Collagenases / metabolism
Humans
Immunohistochemistry
Matrix Metalloproteinase 1
Matrix Metalloproteinase 3 / metabolism
Matrix Metalloproteinase 9
Metalloendopeptidases / metabolism*
Mice
Mice, Nude
Mouth Neoplasms / enzymology*
Mouth Neoplasms / genetics*
Mouth Neoplasms / pathology
Neoplasm Invasiveness / genetics*
Neoplasm Transplantation
Proto-Oncogene Proteins / genetics*
Proto-Oncogene Proteins c-ets
RNA, Antisense / genetics
RNA, Messenger / metabolism
Transcription Factors / genetics*
Transfection / genetics
Tumor Cells, Cultured
|
| IF |
3.491
|
| リソース情報 |
| ヒト・動物細胞 |
MRC-5 |
