論文 - 詳細
| RRC ID | 61322 |
|---|---|
| 著者 | Kamao H, Mandai M, Okamoto S, Sakai N, Suga A, Sugita S, Kiryu J, Takahashi M. |
| タイトル | Characterization of human induced pluripotent stem cell-derived retinal pigment epithelium cell sheets aiming for clinical application. |
| ジャーナル | Stem Cell Reports |
| Abstract |
Age-related macular degeneration (AMD) causes severe visual impairment due in part to age-dependent impairment of retinal pigment epithelium (RPE). It has been suggested that autologous human induced pluripotent stem cells (hiPSCs) may represent a useful cell source for the generation of graft RPE. We generated hiPSC-derived RPE (hiPSC-RPE) cell sheets optimized to meet clinical use requirements, including quality, quantity, consistency, and safety. These cell sheets are generated as a monolayer of cells without any artificial scaffolds, express typical RPE markers, form tight junctions that exhibit polarized secretion of growth factors, and show phagocytotic ability and gene-expression patterns similar to those of native RPE. Additionally, upon transplantation, autologous nonhuman primate iPSC-RPE cell sheets showed no immune rejection or tumor formation. These results suggest that autologous hiPSC-RPE cell sheets may serve as a useful form of graft for use in tissue replacement therapy for AMD. |
| 巻・号 | 2(2) |
| ページ | 205-18 |
| 公開日 | 2014-2-11 |
| DOI | 10.1016/j.stemcr.2013.12.007 |
| PII | S2213-6711(13)00175-6 |
| PMID | 24527394 |
| PMC | PMC3923225 |
| MeSH | Animals Biomarkers / metabolism Cell Culture Techniques* Cell Differentiation Cluster Analysis Epithelial Cells / cytology* Epithelial Cells / immunology Epithelial Cells / metabolism Epithelial Cells / transplantation Gene Expression Profiling Humans Induced Pluripotent Stem Cells / cytology* Macaca fascicularis Rats Retinal Pigment Epithelium / cytology* |
| IF | 6.032 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 114 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 3.0 |
| リソース情報 | |
| ヒト・動物細胞 | 253G1(HPS0002) |