RRC ID 61322
著者 Kamao H, Mandai M, Okamoto S, Sakai N, Suga A, Sugita S, Kiryu J, Takahashi M.
タイトル Characterization of human induced pluripotent stem cell-derived retinal pigment epithelium cell sheets aiming for clinical application.
ジャーナル Stem Cell Reports
Abstract Age-related macular degeneration (AMD) causes severe visual impairment due in part to age-dependent impairment of retinal pigment epithelium (RPE). It has been suggested that autologous human induced pluripotent stem cells (hiPSCs) may represent a useful cell source for the generation of graft RPE. We generated hiPSC-derived RPE (hiPSC-RPE) cell sheets optimized to meet clinical use requirements, including quality, quantity, consistency, and safety. These cell sheets are generated as a monolayer of cells without any artificial scaffolds, express typical RPE markers, form tight junctions that exhibit polarized secretion of growth factors, and show phagocytotic ability and gene-expression patterns similar to those of native RPE. Additionally, upon transplantation, autologous nonhuman primate iPSC-RPE cell sheets showed no immune rejection or tumor formation. These results suggest that autologous hiPSC-RPE cell sheets may serve as a useful form of graft for use in tissue replacement therapy for AMD.
巻・号 2(2)
ページ 205-18
公開日 2014-2-11
DOI 10.1016/j.stemcr.2013.12.007
PII S2213-6711(13)00175-6
PMID 24527394
PMC PMC3923225
MeSH Animals Biomarkers / metabolism Cell Culture Techniques* Cell Differentiation Cluster Analysis Epithelial Cells / cytology* Epithelial Cells / immunology Epithelial Cells / metabolism Epithelial Cells / transplantation Gene Expression Profiling Humans Induced Pluripotent Stem Cells / cytology* Macaca fascicularis Rats Retinal Pigment Epithelium / cytology*
IF 6.032
リソース情報
ヒト・動物細胞 253G1(HPS0002)