論文 - 詳細
| RRC ID | 61424 |
|---|---|
| 著者 | Yuasa K, Hijikata T. |
| タイトル | Distal regulatory element of the STAT1 gene potentially mediates positive feedback control of STAT1 expression. |
| ジャーナル | Genes Cells |
| Abstract |
We previously identified a distal regulatory element located approximately 5.5-kb upstream of the signal transducer and activator of transcription 1 (STAT1) gene, thereafter designating it as 5.5-kb upstream regulatory region (5.5URR). In this study, we investigated the functional roles of 5.5URR in the transcriptional regulation of STAT1 gene. A chromosome conformation capture assay indicated physical interaction of 5.5URR with the STAT1 core promoter. In luciferase reporter assays, 5.5URR-combined STAT1 core promoter exhibited significant increase in reporter activity enhanced by forced STAT1 expression or interferon (IFN) treatment, but STAT1 core promoter alone did not. The 5.5URR contained IFN-stimulated response element and GAS sites, which bound STAT1 complexes in electrophoretic mobility shift assays. Consistently, chromatin immunoprecipitation (ChIP) assays of HEK293 cells with Halo-tagged STAT1 expression indicated the association of Halo-tagged STAT1 with 5.5URR. ChIP assays with IFN treatment demonstrated that IFNs promoted the recruitment of Halo-tagged STAT1 to 5.5URR. Forced STAT1 expression or IFN treatment increased the expression of endogenous STAT1 and other IFN signaling pathway components, such as STAT2, IRF9 and IRF1, besides IFN-responsive genes. Collectively, the results suggest that 5.5URR may provide a regulatory platform for positive feedback control of STAT1 expression possibly to amplify or sustain the intracellular IFN signals. |
| 巻・号 | 21(1) |
| ページ | 25-40 |
| 公開日 | 2016-1-1 |
| DOI | 10.1111/gtc.12316 |
| PMID | 26592235 |
| MeSH | 3T3 Cells Animals Base Sequence Feedback, Physiological* Gene Expression Regulation* Genes, Reporter HEK293 Cells Humans Interferon Regulatory Factor-1 / metabolism Interferons / metabolism Mice Models, Biological Molecular Sequence Data Promoter Regions, Genetic* Protein Binding / genetics STAT1 Transcription Factor / genetics* STAT1 Transcription Factor / metabolism Signal Transduction / genetics Transcription, Genetic Up-Regulation / genetics |
| IF | 1.655 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 2 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | RGB3T3-5(RCB0164) |