論文 - 詳細
| RRC ID | 61450 |
|---|---|
| 著者 | Tabata A, Ohkubo Y, Tamura M, Tomoyasu T, Ohkura K, Nagamune H. |
| タイトル | Construction of an improved drug delivery system tool with enhanced versatility in cell-targeting. |
| ジャーナル | Anticancer Res |
| Abstract |
BACKGROUND/AIM:The aim of this study was to develop an improved drug delivery system (DDS) tool with enhanced versatility in the cell-targeting step using as Z-domain, a modified IgG binding domain of protein A from Staphylococcus aureus, as an IgG adapter domain. MATERIALS AND METHODS:The chimera protein expression system composed of the Z-domain and chimeric cholesterol-dependent cytolysin mutant named His-Z-CDC(ss)(IS) was constructed in Escherichia coli. His-Z-CDC(ss)(IS) was purified by Ni-affinity chromatography, and its abilities for controlled pore formation, membrane binding, IgG binding, and target cell-specific delivery of liposomes carrying medicine were investigated. RESULTS AND DISCUSSION:His-Z-CDC(ss)(IS) purified by Ni-affinity chromatography indicated pore-forming activity only under disulfide bond reducing conditions. His-Z-CDC(ss)(IS) also demonstrated an ability to bind both IgG and cholesterol-embedded liposomes via its Z-domain and domain 4, respectively. Furthermore, anticarcinoembryonic antigen (CEA) IgG-bound His-Z-CDC(ss)(IS) indicated effective delivery of liposomes carrying drugs to CEA-expressing cells. CONCLUSION:His-Z-CDC(ss)(IS) was revealed to be an improved DDS tool with enhanced versatility in cell targeting. |
| 巻・号 | 33(7) |
| ページ | 2905-10 |
| 公開日 | 2013-7-1 |
| PII | 33/7/2905 |
| PMID | 23780978 |
| MeSH | Carcinoembryonic Antigen / metabolism* Cell Membrane / drug effects Cell Membrane / metabolism Cells, Cultured Cholesterol / metabolism Drug Delivery Systems* Fibroblasts / metabolism* Fluorouracil / administration & dosage Hemolysis / drug effects Hep G2 Cells / metabolism* Humans Immunoglobulin G / metabolism Liposomes* Perforin / genetics Perforin / metabolism* Protein Transport Recombinant Fusion Proteins / metabolism* Staphylococcus aureus |
| IF | 1.994 |
| リソース情報 | |
| ヒト・動物細胞 | NB1RGB(RCB0222) |