RRC ID 6147
Author Kato M, Nagasaki-Takeuchi N, Ide Y, Maeshima M.
Title An Arabidopsis hydrophilic Ca2(+) -binding protein with a PEVK-rich domain, PCaP2, is associated with the plasma membrane and interacts with calmodulin and phosphatidylinositol phosphates.
Journal Plant Cell Physiol
Abstract We found a new hydrophilic protein in Arabidopsis thaliana. Real-time PCR demonstrated that the protein was expressed in roots. Histochemical analysis of promoter-beta-glucuronidase fusions demonstrated its extensive expression in root hairs. The protein is rich in proline, glutamate, valine and lysine residues (PEVK-rich domain), and bound Ca(2+) even in the presence of Mg(2+) and K(+) when examined by the (45)Ca overlay assay. Treatment of seedlings with K(+), Mn(2+), Zn(2+), Na(+), ABA and gibberellic acid, and cold and drought stresses enhanced the transcription. Expression of the protein linked to green fluorescent protein in A. thaliana showed its plasma membrane localization and cell-specific expression in the epidermal cells including root hairs and the elongating pollen tubes. Therefore, we named the protein PCaP2 (plasma membrane-associated Ca(2+)-binding protein-2). The substitution of glycine at position 2 with alanine resulted in cytoplasmic localization of PCaP2. These results and the N-terminal characteristic motif suggest that PCaP2 is N-myristoylated at Gly2. We examined the capacity for binding to phosphatidylinositol phosphates (PtdInsPs), and found that PCaP2 interacts strongly with PtdIns(3,5)P(2), PtdIns(4,5)P(2) and PtdIns(3,4,5)P(3), and weakly with PtdIns(3,4)P(2). Furthermore, calmodulin was associated with PCaP2 in a Ca(2+)-dependent manner, and its association weakened the interaction of PCaP2 with PtdInsPs. These results indicate that PCaP2 is involved in intracellular signaling through interaction with PtdInsPs and calmodulin in growing root hairs. PCaP2 was previously reported as microtubule-associated protein-18. We discuss the physiological roles of PCaP2 in relation to microtubules in cells.
Volume 51(3)
Pages 366-79
Published 2010-3-1
DOI 10.1093/pcp/pcq003
PII pcq003
PMID 20061304
MeSH Amino Acid Sequence Arabidopsis / genetics* Arabidopsis / metabolism Arabidopsis Proteins / genetics Arabidopsis Proteins / metabolism* Calcium Signaling Calcium-Binding Proteins / genetics Calcium-Binding Proteins / metabolism* Calmodulin / metabolism* Cell Membrane / metabolism Gene Expression Regulation, Plant Molecular Sequence Data Phosphatidylinositol Phosphates / metabolism* Plant Roots / genetics Plant Roots / metabolism Plants, Genetically Modified / genetics Plants, Genetically Modified / metabolism RNA, Plant / genetics Sequence Alignment
IF 4.062
Times Cited 36
WOS Category PLANT SCIENCES CELL BIOLOGY
Resource
Arabidopsis / Cultured plant cells, genes sja05800