Reference - Detail
| RRC ID | 61620 |
|---|---|
| Author | Takayama Y, Kitsunai K, Mizumachi K. |
| Title | Factors in bovine colostrum that enhance the migration of human fibroblasts in type I collagen gels. |
| Journal | Biosci Biotechnol Biochem |
| Abstract |
Bovine colostrum has an activity that increases the migration of WI38 fibroblasts. We evaluated the motility of fibroblasts by their ability to contract collagen gels. Part of the activity was absorbed by anion-exchange chromatography at pH 6.4, and eluted by 0.2-0.3 M sodium chloride. The activity was separated into many fractions corresponding to 20-150 kDa by gel filtration chromatography under acidic conditions. The major peak of the activity coincided with 50-70 kDa. |
| Volume | 65(12) |
| Pages | 2776-9 |
| Published | 2001-12-1 |
| DOI | 10.1271/bbb.65.2776 |
| PMID | 11826977 |
| MeSH | Animals Anion Exchange Resins Cattle Cell Movement / physiology* Chromatography, Gel Chromatography, Ion Exchange Collagen Type I / metabolism* Colostrum / physiology* Fibroblasts / cytology* Humans Hydrogen-Ion Concentration |
| IF | 1.516 |
| Resource | |
| Human and Animal Cells | WI-38 |