論文 - 詳細
| RRC ID | 6164 |
|---|---|
| 著者 | Haselier A, Akbari H, Weth A, Baumgartner W, Frentzen M. |
| タイトル | Two closely related genes of Arabidopsis encode plastidial cytidinediphosphate diacylglycerol synthases essential for photoautotrophic growth. |
| ジャーナル | Plant Physiol |
| Abstract |
Cytidinediphosphate diacylglycerol synthase (CDS) catalyzes the formation of cytidinediphosphate diacylglycerol, an essential precursor of anionic phosphoglycerolipids like phosphatidylglycerol or -inositol. In plant cells, CDS isozymes are located in plastids, mitochondria, and microsomes. Here, we show that these isozymes are encoded by five genes in Arabidopsis (Arabidopsis thaliana). Alternative translation initiation or alternative splicing of CDS2 and CDS4 transcripts can result in up to 10 isoforms. Most of the cDNAs encoding the various plant isoforms were functionally expressed in yeast and rescued the nonviable phenotype of the mutant strain lacking CDS activity. The closely related genes CDS4 and CDS5 were found to encode plastidial isozymes with similar catalytic properties. Inactivation of both genes was required to obtain Arabidopsis mutant lines with a visible phenotype, suggesting that the genes have redundant functions. Analysis of these Arabidopsis mutants provided further independent evidence for the importance of plastidial phosphatidylglycerol for structure and function of thylakoid membranes and, hence, for photoautotrophic growth. |
| 巻・号 | 153(3) |
| ページ | 1372-84 |
| 公開日 | 2010-7-1 |
| DOI | 10.1104/pp.110.156422 |
| PII | pp.110.156422 |
| PMID | 20442275 |
| PMC | PMC2899908 |
| MeSH | Alleles Arabidopsis / enzymology Arabidopsis / genetics* Arabidopsis / growth & development* Arabidopsis / ultrastructure Autotrophic Processes / drug effects Autotrophic Processes / genetics Autotrophic Processes / radiation effects* DNA, Bacterial / genetics Diacylglycerol Cholinephosphotransferase / genetics* Diacylglycerol Cholinephosphotransferase / metabolism Genes, Plant / genetics* Genetic Complementation Test Glycerophospholipids / biosynthesis Isoenzymes / genetics Isoenzymes / metabolism Light* Membrane Lipids / metabolism Mutagenesis, Insertional / drug effects Mutagenesis, Insertional / genetics Mutagenesis, Insertional / radiation effects Mutation / genetics Phenotype Plastids / enzymology* Plastids / genetics Plastids / radiation effects Plastids / ultrastructure Protein Transport / drug effects Protein Transport / radiation effects Saccharomyces cerevisiae / cytology Saccharomyces cerevisiae / drug effects Saccharomyces cerevisiae / metabolism Saccharomyces cerevisiae / radiation effects Subcellular Fractions / drug effects Subcellular Fractions / enzymology Subcellular Fractions / radiation effects Sucrose / pharmacology |
| IF | 6.902 |
| 引用数 | 29 |
| WOS 分野 | PLANT SCIENCES |
| リソース情報 | |
| シロイヌナズナ / 植物培養細胞・遺伝子 | pda06248 pda03915 pda01154 |