| RRC ID |
6167
|
| 著者 |
Arent S, Christensen CE, Pye VE, Nørgaard A, Henriksen A.
|
| タイトル |
The multifunctional protein in peroxisomal beta-oxidation: structure and substrate specificity of the Arabidopsis thaliana protein MFP2.
|
| ジャーナル |
J Biol Chem
|
| Abstract |
Plant fatty acids can be completely degraded within the peroxisomes. Fatty acid degradation plays a role in several plant processes including plant hormone synthesis and seed germination. Two multifunctional peroxisomal isozymes, MFP2 and AIM1, both with 2-trans-enoyl-CoA hydratase and l-3-hydroxyacyl-CoA dehydrogenase activities, function in mouse ear cress (Arabidopsis thaliana) peroxisomal beta-oxidation, where fatty acids are degraded by the sequential removal of two carbon units. A deficiency in either of the two isozymes gives rise to a different phenotype; the biochemical and molecular background for these differences is not known. Structure determination of Arabidopsis MFP2 revealed that plant peroxisomal MFPs can be grouped into two families, as defined by a specific pattern of amino acid residues in the flexible loop of the acyl-binding pocket of the 2-trans-enoyl-CoA hydratase domain. This could explain the differences in substrate preferences and specific biological functions of the two isozymes. The in vitro substrate preference profiles illustrate that the Arabidopsis AIM1 hydratase has a preference for short chain acyl-CoAs compared with the Arabidopsis MFP2 hydratase. Remarkably, neither of the two was able to catabolize enoyl-CoA substrates longer than 14 carbon atoms efficiently, suggesting the existence of an uncharacterized long chain enoyl-CoA hydratase in Arabidopsis peroxisomes.
|
| 巻・号 |
285(31)
|
| ページ |
24066-77
|
| 公開日 |
2010-7-30
|
| DOI |
10.1074/jbc.M110.106005
|
| PII |
S0021-9258(20)61873-7
|
| PMID |
20463021
|
| PMC |
PMC2911295
|
| MeSH |
Arabidopsis / enzymology*
Arabidopsis Proteins / chemistry*
Arabidopsis Proteins / metabolism
Crystallography, X-Ray / methods
Fatty Acids / chemistry
Gene Expression Regulation, Plant*
Models, Biological
Oxidation-Reduction
Oxygen / chemistry*
Peroxisomes / chemistry*
Phenotype
Protein Binding
Protein Conformation
Protein Isoforms
Protein Structure, Tertiary
Substrate Specificity
|
| IF |
4.238
|
| 引用数 |
25
|
|
WOS 分野
|
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| リソース情報 |
| シロイヌナズナ / 植物培養細胞・遺伝子 |
pda05150
pda08625
pda02497 |
