論文 - 詳細
| RRC ID | 61819 |
|---|---|
| 著者 | Fukuda T, Ochi H, Sunamura S, Haiden A, Bando W, Inose H, Okawa A, Asou Y, Takeda S. |
| タイトル | MicroRNA-145 regulates osteoblastic differentiation by targeting the transcription factor Cbfb. |
| ジャーナル | FEBS Lett |
| Abstract |
Osteoblastic differentiation is regulated by various factors, including hormones and transcription factors. Runt-related transcription factor 2 (Runx2) is an essential player in osteoblastogenesis and transactivates its molecular target by creating a protein complex with its hetero-dimeric partner core binding factor beta (Cbfb). However, the molecular regulation of Cbfb expression remains unknown. Here, we identified miR-145 as a crucial regulator of Cbfb expression. The expression of miR-145 increased during osteoblastogenesis, indicating that miR-145 works as an inhibitor of osteoblastogenesis. Stable expression of miR-145 decreased endogenous Cbfb expression and inhibited osteoblastogenesis, in cooperation with miR-34c. Furthermore, miR-145 decreased bone regeneration in vivo. Our results indicate that miR-145 physiologically regulates osteoblast differentiation and bone formation via Cbfb expression by forming a regulatory microRNA network. |
| 巻・号 | 589(21) |
| ページ | 3302-8 |
| 公開日 | 2015-10-24 |
| DOI | 10.1016/j.febslet.2015.09.024 |
| PII | S0014-5793(15)00873-X |
| PMID | 26450370 |
| MeSH | 3T3 Cells Animals Bone Regeneration Cell Differentiation Cell Line Core Binding Factor beta Subunit / genetics* Core Binding Factor beta Subunit / metabolism Gene Expression Regulation HEK293 Cells Humans Mice MicroRNAs / metabolism* Osteoblasts / physiology* Promoter Regions, Genetic |
| IF | 3.057 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 2 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | UCB408E6E7TERT-33(RCB2080) GE1(RCB1709) |