RRC ID 6182
Author Lee JH, Shin SY, Kim S, Choo J, Lee YH.
Title Suppression of PTEN expression during aggregation with retinoic acid in P19 mouse embryonal carcinoma cells.
Journal Biochem Biophys Res Commun
Abstract Apoptosis is thought to be involved in the maintenance of cellular homeostasis, as well as various pathological processes. However, little information is available about the regulation of apoptosis during the aggregation stage of P19 embryonal carcinoma (EC) cells. Here we report that aggregation-induced apoptosis is markedly attenuated by treatment with retinoic acid (RA). PTEN (phosphatase and tensin homolog deleted on chromosome 10) expression was down-regulated during the aggregation phase of P19 EC cells in the presence, but not in the absence, of RA. Suppression of PTEN expression during the aggregation was accompanied by increased phosphorylation of serine/threonine kinase Akt and glycogen synthase kinase-3beta (GSK-3beta). Our results suggest that RA attenuates the induction of apoptosis during the aggregation phase of P19 EC cells, probably by suppressing PTEN expression.
Volume 347(3)
Pages 715-22
Published 2006-9-1
DOI 10.1016/j.bbrc.2006.06.161
PII S0006-291X(06)01464-1
PMID 16842746
MeSH Animals Apoptosis / drug effects Carcinoma, Embryonal / genetics Carcinoma, Embryonal / metabolism* Carcinoma, Embryonal / pathology Cell Differentiation Cell Shape / drug effects Down-Regulation / drug effects Enzyme Activation / drug effects Gene Expression Regulation, Neoplastic Mice PTEN Phosphohydrolase / genetics PTEN Phosphohydrolase / metabolism* Poly(ADP-ribose) Polymerases / metabolism Proto-Oncogene Proteins c-akt / metabolism Transcription, Genetic / genetics Tretinoin / pharmacology*
IF 2.985
Times Cited 15
DNA material AxCAhPTEN1 (RDB02012) AxCALacZ (RDB01745).