RRC ID 61854
著者 Le NQ, Binh NT, Takarada T, Takarada-Iemata M, Hinoi E, Yoneda Y.
タイトル Negative correlation between Per1 and Sox6 expression during chondrogenic differentiation in pre-chondrocytic ATDC5 cells.
ジャーナル J Pharmacol Sci
Abstract Pre-chondrocytes undergo cellular differentiation stages during chondrogenesis under the influence by different transcription factors such as sry-type high mobility group box-9 (Sox9) and runt-related transcription factor-2 (Runx2). We have shown upregulation by parathyroid hormone (PTH) of the clock gene Period-1 (Per1) through the cAMP/protein kinase A signaling pathway in pre-chondrocytic ATDC5 cells. Here, we investigated the role of Per1 in the suppression of chondrogenic differentiation by PTH. In ATDC5 cells exposed to 10 nM PTH, a drastic but transient increase in Per1 expression was seen only 1 h after addition together with a prolonged decrease in Sox6 levels. However, no significant changes were induced in Sox5 and Runx2 levels in cells exposed to PTH. In stable Per1 transfectants, a significant decrease in Sox6 levels was seen, with no significant changes in Sox5 and Sox9 levels, in addition to the inhibition of gene transactivation by Sox9 allies. Knockdown of Per1 by siRNA significantly increased the Sox6 and type II collagen levels in cells cultured for 24 - 60 h. These results suggest that Per1 plays a role in the suppressed chondrocytic differentiation by PTH through a mechanism relevant to negative regulation of transactivation of the Sox6 gene during chondrogenesis.
巻・号 122(4)
ページ 318-25
公開日 2013-1-1
DOI 10.1254/jphs.13091fp
PII DN/JST.JSTAGE/jphs/13091FP
PMID 23883486
MeSH Animals Cell Differentiation / drug effects Cell Differentiation / genetics* Cells, Cultured Chondrocytes / cytology* Chondrogenesis / drug effects Chondrogenesis / genetics* Cyclic AMP-Dependent Protein Kinases / physiology Gene Expression Regulation, Developmental / drug effects Gene Expression Regulation, Developmental / genetics* Mice Parathyroid Hormone / pharmacology Period Circadian Proteins / genetics* Period Circadian Proteins / metabolism Period Circadian Proteins / physiology* RNA, Small Interfering SOXD Transcription Factors / genetics* SOXD Transcription Factors / metabolism SOXD Transcription Factors / physiology* Signal Transduction / genetics Signal Transduction / physiology Transcriptional Activation / genetics
IF 2.835
リソース情報
ヒト・動物細胞 ATDC5(RCB0565)