RRC ID 61876
Author Kaito Y, Kataoka R, Takechi K, Mihara T, Tamura M.
Title Nox1 activation by βPix and the role of Ser-340 phosphorylation.
Journal FEBS Lett
Abstract Rac is an activating factor for Nox1, an O2(-)-generating NADPH oxidase, expressed in the colon and other tissues. Rac requires a GDP-GTP exchange factor for activation. Nox1 activation by βPix has been demonstrated in cell lines. We examined the effects of βPix and its phosphomimetic mutant on endogenous Nox1 in Caco-2 cells transfected with Noxo1 and Noxa1. βPix expression enhanced O2(-) production in resting cells and cells stimulated with EGF or phorbol ester. βPix(S340E) further enhanced O2(-) production, while βPix(S340A) eliminated the βPix effect. βPix(S340E), but not βPix(S340A), had higher affinity and GEF activity for Rac than wild-type βPix. These results suggest that βPix phosphorylation at Ser-340 upregulates Nox1 through Rac activation, confirming Rac as a trigger for acute Nox1-dependent ROS production.
Volume 588(11)
Pages 1997-2002
Published 2014-5-29
DOI 10.1016/j.febslet.2014.04.025
PII S0014-5793(14)00311-1
PMID 24792722
MeSH Adaptor Proteins, Vesicular Transport / metabolism Amino Acid Sequence Amino Acid Substitution Caco-2 Cells Enzyme Activation Epidermal Growth Factor / physiology Guanosine Triphosphate / chemistry Humans Hydrolysis Mutagenesis, Site-Directed NADPH Oxidase 1 NADPH Oxidases / metabolism* Phosphorylation Protein Binding Protein Processing, Post-Translational* Rho Guanine Nucleotide Exchange Factors / chemistry Rho Guanine Nucleotide Exchange Factors / genetics Rho Guanine Nucleotide Exchange Factors / metabolism* Serine / metabolism Superoxides / metabolism rac GTP-Binding Proteins / chemistry
IF 3.057
Human and Animal Cells CACO-2(RCB0988)