論文 - 詳細
| RRC ID | 61876 |
|---|---|
| 著者 | Kaito Y, Kataoka R, Takechi K, Mihara T, Tamura M. |
| タイトル | Nox1 activation by βPix and the role of Ser-340 phosphorylation. |
| ジャーナル | FEBS Lett |
| Abstract |
Rac is an activating factor for Nox1, an O2(-)-generating NADPH oxidase, expressed in the colon and other tissues. Rac requires a GDP-GTP exchange factor for activation. Nox1 activation by βPix has been demonstrated in cell lines. We examined the effects of βPix and its phosphomimetic mutant on endogenous Nox1 in Caco-2 cells transfected with Noxo1 and Noxa1. βPix expression enhanced O2(-) production in resting cells and cells stimulated with EGF or phorbol ester. βPix(S340E) further enhanced O2(-) production, while βPix(S340A) eliminated the βPix effect. βPix(S340E), but not βPix(S340A), had higher affinity and GEF activity for Rac than wild-type βPix. These results suggest that βPix phosphorylation at Ser-340 upregulates Nox1 through Rac activation, confirming Rac as a trigger for acute Nox1-dependent ROS production. |
| 巻・号 | 588(11) |
| ページ | 1997-2002 |
| 公開日 | 2014-5-29 |
| DOI | 10.1016/j.febslet.2014.04.025 |
| PII | S0014-5793(14)00311-1 |
| PMID | 24792722 |
| MeSH | Adaptor Proteins, Signal Transducing Adaptor Proteins, Vesicular Transport / metabolism Amino Acid Sequence Amino Acid Substitution Caco-2 Cells Enzyme Activation Epidermal Growth Factor / physiology Guanosine Triphosphate / chemistry Humans Hydrolysis Mutagenesis, Site-Directed NADPH Oxidase 1 NADPH Oxidases / metabolism* Phosphorylation Protein Binding Protein Processing, Post-Translational* Rho Guanine Nucleotide Exchange Factors / chemistry Rho Guanine Nucleotide Exchange Factors / genetics Rho Guanine Nucleotide Exchange Factors / metabolism* Serine / metabolism Superoxides / metabolism rac GTP-Binding Proteins / chemistry |
| IF | 3.057 |
| リソース情報 | |
| ヒト・動物細胞 | CACO-2(RCB0988) |