RRC ID 61974
Author Usami N, Maeda M, Eguchi-Kasai K, Maezawa H, Kobayashi K.
Title Radiation-induced gamma-H2AX in mammalian cells irradiated with a synchrotron X-ray microbeam.
Journal Radiat Prot Dosimetry
Abstract In order to study the radiobiological effects from low dose radiation, a cell irradiation system using synchrotron X-ray microbeam has been developed, by which cells can be recognised individually and irradiated one by one with the desired dose of monochromatic X rays. The minimum beam sizes obtained are 2 microm with the focusing optics and 5 microm square with the non-focused beam, and the beam size can be changed easily with a high-precision slit in the case of a non-focused beam. Human fibroblast cells were individually irradiated with this system, and immunostained by gamma-H2AX antibody to visualise the DNA damage. Most of the fluorescent foci were observed in a localised area in cell nuclei, the size of which was almost the same as the beam size.
Volume 122(1-4)
Pages 307-9
Published 2006-1-1
DOI 10.1093/rpd/ncl434
PII ncl434
PMID 17182605
MeSH Cells, Cultured DNA / genetics* DNA / radiation effects* DNA Damage* Dose-Response Relationship, Radiation Equipment Design Equipment Failure Analysis Fibroblasts / physiology* Fibroblasts / radiation effects* Histones / genetics* Humans Miniaturization Radiation Dosage Synchrotrons / instrumentation* X-Rays
IF 0.773
Human and Animal Cells NB1RGB(RCB0222)