| RRC ID |
61974
|
| 著者 |
Usami N, Maeda M, Eguchi-Kasai K, Maezawa H, Kobayashi K.
|
| タイトル |
Radiation-induced gamma-H2AX in mammalian cells irradiated with a synchrotron X-ray microbeam.
|
| ジャーナル |
Radiat Prot Dosimetry
|
| Abstract |
In order to study the radiobiological effects from low dose radiation, a cell irradiation system using synchrotron X-ray microbeam has been developed, by which cells can be recognised individually and irradiated one by one with the desired dose of monochromatic X rays. The minimum beam sizes obtained are 2 microm with the focusing optics and 5 microm square with the non-focused beam, and the beam size can be changed easily with a high-precision slit in the case of a non-focused beam. Human fibroblast cells were individually irradiated with this system, and immunostained by gamma-H2AX antibody to visualise the DNA damage. Most of the fluorescent foci were observed in a localised area in cell nuclei, the size of which was almost the same as the beam size.
|
| 巻・号 |
122(1-4)
|
| ページ |
307-9
|
| 公開日 |
2006-1-1
|
| DOI |
10.1093/rpd/ncl434
|
| PII |
ncl434
|
| PMID |
17182605
|
| MeSH |
Cells, Cultured
DNA / genetics*
DNA / radiation effects*
DNA Damage*
Dose-Response Relationship, Radiation
Equipment Design
Equipment Failure Analysis
Fibroblasts / physiology*
Fibroblasts / radiation effects*
Histones / genetics*
Humans
Miniaturization
Radiation Dosage
Synchrotrons / instrumentation*
X-Rays
|
| IF |
0.773
|
| リソース情報 |
| ヒト・動物細胞 |
NB1RGB(RCB0222) |
