RRC ID 61981
Author Nagai M, Sato N.
Title Reciprocal gene expression of osteoclastogenesis inhibitory factor and osteoclast differentiation factor regulates osteoclast formation.
Journal Biochem Biophys Res Commun
Abstract Osteoblasts/stromal cells support the formation of osteoclast-like cells (OCL) from osteoclast progenitor cells via expressing a membrane-associated protein, osteoclast differentiation factor (ODF), in the presence of osteotropic factors, whereas the cells secrete a substantial amount of osteoclastogenesis inhibitory factor (OCIF) in the unstimulated state. There are both OCL formation-supporting and the nonsupporting cell lines in osteoblasts/stromal cell lineages. The mechanism that divides osteoblasts/stromal cell lines into the two types is not known. The present study reports that OCL formation-supporting cell line ST2 showed a greatly increased level of ODF mRNA, whereas their OCIF mRNA was drastically diminished in the presence of 1alpha, 25(OH)2-dihydroxyvitamin D3 or prostaglandin E2. In contrast, MC3T3-E1 cells lacking OCL formation-supporting ability did not show a decrease in OCIF mRNA in response to the factors, despite a similar increase in ODF mRNA as ST2 cells. However, inactivated MC3T3-E1 cells secreting nothing supported OCL formation in coculture with human promyelocytic cells, HL60. On the contrary, ST2 cells did not support OCL formation from HL60 cells when cocultured in medium conditioned by 1alpha, 25(OH)2 vitamin D3-treated MC3T3-E1. These findings indicate that reciprocal gene expression of ODF and OCIF in osteoblasts/stromal cells is essential for supporting OCL formation.
Volume 257(3)
Pages 719-23
Published 1999-4-21
DOI 10.1006/bbrc.1999.0524
PII S0006-291X(99)90524-7
PMID 10208850
MeSH Animals Calcitriol / pharmacology Carrier Proteins / genetics* Carrier Proteins / physiology Cell Differentiation / drug effects Cell Line Cell Membrane / metabolism Coculture Techniques Culture Media, Conditioned / pharmacology Dexamethasone / pharmacology Dinoprostone / pharmacology Gene Expression Regulation* / drug effects Glycoproteins / genetics* Glycoproteins / physiology HL-60 Cells Humans Membrane Glycoproteins / genetics* Membrane Glycoproteins / physiology Mice Osteoblasts / cytology Osteoblasts / drug effects Osteoblasts / metabolism* Osteoclasts / cytology* Osteoclasts / drug effects Osteoclasts / metabolism Osteoprotegerin RANK Ligand RNA, Messenger / metabolism Receptor Activator of Nuclear Factor-kappa B Receptors, Calcitonin / genetics Receptors, Cytoplasmic and Nuclear* Receptors, Tumor Necrosis Factor / genetics Stem Cells / cytology Stem Cells / drug effects Stem Cells / metabolism Stromal Cells / cytology Stromal Cells / drug effects Stromal Cells / metabolism Tissue Fixation
IF 2.985
Human and Animal Cells HL60(RCB0041) MC3T3-E1(RCB1126) MC3T3-G2/PA6(RCB1127) ST2(RCB0224)