論文 - 詳細
| RRC ID | 62011 |
|---|---|
| 著者 | Odkhuu E, Komatsu T, Naiki Y, Koide N, Yokochi T. |
| タイトル | Sendai virus C protein inhibits lipopolysaccharide-induced nitric oxide production through impairing interferon-β signaling. |
| ジャーナル | Int Immunopharmacol |
| Abstract |
The effect of Sendai virus (SeV) C protein on lipopolysaccharide (LPS)-induced nitric oxide (NO) production was examined using RAW 264.7 macrophage cells. Infection of SeV inhibited LPS-induced NO production via downregulating the expression of an inducible NO synthase protein (iNOS). On the other hand, C gene-knockout 4C(-) SeV inhibited neither NO production nor iNOS expression. Wild type and 4C(-) SeV did not affect LPS-induced production of tumor necrosis factor-α and interleukin-6, and further LPS-induced activation of nuclear factor (NF)-κB and mitogen-activated protein kinases. Although wild type and 4C(-) SeV did not inhibit LPS-induced interferon (IFN)-β production, wild type SeV but not 4C(-) SeV inhibited the activation of STAT1/2 in the IFN-β signaling. SeV C protein inhibited LPS-induced iNOS expression and NO production. C protein inhibited the promotor activation of IFN-β and IFN-sensitive response element (ISRE) in response to LPS whereas the C mutant protein CF170S, which lacks the ability to block the STAT activation, did not inhibit it. Taken together, SeV C protein was suggested to inhibit LPS-induced NO production through impairing IFN-β signaling. |
| 巻・号 | 23(1) |
| ページ | 267-72 |
| 公開日 | 2014-11-1 |
| DOI | 10.1016/j.intimp.2014.09.012 |
| PII | S1567-5769(14)00364-6 |
| PMID | 25242386 |
| MeSH | Animals Cell Line Gene Expression Regulation, Viral / genetics Gene Knockout Techniques Interferon-beta / metabolism* Lipopolysaccharides / metabolism Macrophages / immunology* Macrophages / virology Mice Mutation / genetics Nitric Oxide / metabolism Nitric Oxide Synthase Type II / genetics Nitric Oxide Synthase Type II / metabolism Respirovirus Infections / immunology* STAT1 Transcription Factor / metabolism STAT2 Transcription Factor / metabolism Sendai virus / physiology* Signal Transduction / genetics Transcriptional Activation / genetics Viral Proteins / genetics Viral Proteins / metabolism* |
| IF | 3.943 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Wikipedia |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | RAW 264(RCB0535) |