論文 - 詳細
| RRC ID | 62071 |
|---|---|
| 著者 | Izumi M, Yanagi K, Mizuno T, Yokoi M, Kawasaki Y, Moon KY, Hurwitz J, Yatagai F, Hanaoka F. |
| タイトル | The human homolog of Saccharomyces cerevisiae Mcm10 interacts with replication factors and dissociates from nuclease-resistant nuclear structures in G(2) phase. |
| ジャーナル | Nucleic Acids Res |
| Abstract |
Mcm10 (Dna43), first identified in Saccharomyces cerevisiae, is an essential protein which functions in the initiation of DNA synthesis. Mcm10 is a nuclear protein that is localized to replication origins and mediates the interaction of the Mcm2-7 complex with replication origins. We identified and cloned a human cDNA whose product was structurally homologous to the yeast Mcm10 protein. Human Mcm10 (HsMcm10) is a 98-kDa protein of 874 amino acids which shows 23 and 21% overall similarity to Schizosaccharomyces pombe Cdc23 and S. cerevisiae Mcm10, respectively. The messenger RNA level of HsMcm10 increased at the G(1)/S-boundary when quiescent human NB1-RGB cells were induced to proliferate as is the case of many replication factors. HsMcm10 associated with nuclease-resistant nuclear structures throughout S phase and dissociated from it in G(2) phase. HsMcm10 associated with human Orc2 protein when overexpressed in COS-1 cells. HsMcm10 also interacted with Orc2, Mcm2 and Mcm6 proteins in the yeast two-hybrid system. These results suggest that HsMcm10 may function in DNA replication through the interaction with Orc and Mcm2-7 complexes. |
| 巻・号 | 28(23) |
| ページ | 4769-77 |
| 公開日 | 2000-12-1 |
| DOI | 10.1093/nar/28.23.4769 |
| PMID | 11095689 |
| PMC | PMC115166 |
| MeSH | Amino Acid Sequence Animals COS Cells Cell Cycle / genetics Cell Cycle Proteins / genetics* Cell Cycle Proteins / metabolism Cell Line Cell Nucleus / metabolism* Chromosomal Proteins, Non-Histone DNA Replication DNA, Complementary / chemistry DNA, Complementary / genetics DNA, Recombinant DNA-Binding Proteins / genetics DNA-Binding Proteins / metabolism* Deoxyribonucleases / metabolism* G2 Phase* Gene Expression HeLa Cells Humans Minichromosome Maintenance Proteins Molecular Sequence Data Origin Recognition Complex Plasmids / genetics Precipitin Tests Protein Binding RNA, Messenger / genetics RNA, Messenger / metabolism Saccharomyces cerevisiae / genetics Saccharomyces cerevisiae Proteins* Schizosaccharomyces pombe Proteins* Sequence Alignment Sequence Analysis, DNA Sequence Homology, Amino Acid Two-Hybrid System Techniques |
| IF | 11.502 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Wikipedia |
| 各媒体での言及数の合計 | 7 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | NB1RGB(RCB0222) |
| 遺伝子材料 | Human MCM10 cDNA (RDB06320) |