論文 - 詳細
| RRC ID | 62080 |
|---|---|
| 著者 | Masutani C, Kusumoto R, Yamada A, Dohmae N, Yokoi M, Yuasa M, Araki M, Iwai S, Takio K, Hanaoka F. |
| タイトル | The XPV (xeroderma pigmentosum variant) gene encodes human DNA polymerase eta. |
| ジャーナル | Nature |
| Abstract |
Xeroderma pigmentosum variant (XP-V) is an inherited disorder which is associated with increased incidence of sunlight-induced skin cancers. Unlike other xeroderma pigmentosum cells (belonging to groups XP-A to XP-G), XP-V cells carry out normal nucleotide-excision repair processes but are defective in their replication of ultraviolet-damaged DNA. It has been suspected for some time that the XPV gene encodes a protein that is involved in trans-lesion DNA synthesis, but the gene product has never been isolated. Using an improved cell-free assay for trans-lesion DNA synthesis, we have recently isolated a DNA polymerase from HeLa cells that continues replication on damaged DNA by bypassing ultraviolet-induced thymine dimers in XP-V cell extracts. Here we show that this polymerase is a human homologue of the yeast Rad30 protein, recently identified as DNA polymerase eta. This polymerase and yeast Rad30 are members of a family of damage-bypass replication proteins which comprises the Escherichia coli proteins UmuC and DinB and the yeast Rev1 protein. We found that all XP-V cells examined carry mutations in their DNA polymerase eta gene. Recombinant human DNA polymerase eta corrects the inability of XP-V cell extracts to carry out DNA replication by bypassing thymine dimers on damaged DNA. Together, these results indicate that DNA polymerase eta could be the XPV gene product. |
| 巻・号 | 399(6737) |
| ページ | 700-4 |
| 公開日 | 1999-6-17 |
| DOI | 10.1038/21447 |
| PMID | 10385124 |
| MeSH | Alleles Amino Acid Sequence Animals Cell Line Cell-Free System Cloning, Molecular DNA Polymerase iota DNA Repair DNA-Directed DNA Polymerase / genetics* HeLa Cells Humans Molecular Sequence Data Mutation Recombinant Proteins / genetics Recombinant Proteins / metabolism Saccharomyces cerevisiae / enzymology Sequence Homology, Amino Acid Xeroderma Pigmentosum / enzymology Xeroderma Pigmentosum / genetics* |
| IF | 42.779 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 21 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | NB1RGB(RCB0222) |