RRC ID 62112
著者 Divya MS, Roshin GE, Divya TS, Rasheed VA, Santhoshkumar TR, Elizabeth KE, James J, Pillai RM.
タイトル Umbilical cord blood-derived mesenchymal stem cells consist of a unique population of progenitors co-expressing mesenchymal stem cell and neuronal markers capable of instantaneous neuronal differentiation.
ジャーナル Stem Cell Res Ther
Abstract INTRODUCTION:Umbilical cord blood (UCB)-derived mesenchymal stem cells (MSCs) are self-renewing multipotent progenitors with the potential to differentiate into multiple lineages of mesoderm, in addition to generating ectodermal and endodermal lineages by crossing the germline barrier. In the present study we have investigated the ability of UCB-MSCs to generate neurons, since we were able to observe varying degrees of neuronal differentiation from a few batches of UCB-MSCs with very simple neuronal induction protocols whereas other batches required extensive exposure to combination of growth factors in a stepwise protocol. Our hypothesis was therefore that the human UCB-MSCs would contain multiple types of progenitors with varying neurogenic potential and that the ratio of the progenitors with high and low neurogenic potentials varies in different batches of UCB.
METHODS:In total we collected 45 UCB samples, nine of which generated MSCs that were further expanded and characterized using immunofluorescence, fluorescence-activated cell sorting and RT-PCR analysis. The neuronal differentiation potential of the UCB-MSCs was analyzed with exposure to combination of growth factors.
RESULTS:We could identify two different populations of progenitors within the UCB-MSCs. One population represented progenitors with innate neurogenic potential that initially express pluripotent stem cell markers such as Oct4, Nanog, Sox2, ABCG2 and neuro-ectodermal marker nestin and are capable of expanding and differentiating into neurons with exposure to simple neuronal induction conditions. The remaining population of cells, typically expressing MSC markers, requires extensive exposure to a combination of growth factors to transdifferentiate into neurons. Interesting to note was that both of these cell populations were positive for CD29 and CD105, indicating their MSC lineage, but showed prominent difference in their neurogenic potential.
CONCLUSION:Our results suggest that the expanded UCB-derived MSCs harbor a small unique population of cells that express pluripotent stem cell markers along with MSC markers and possess an inherent neurogenic potential. These pluripotent progenitors later generate cells expressing neural progenitor markers and are responsible for the instantaneous neuronal differentiation; the ratio of these pluripotent marker expressing cells in a batch determines the innate neurogenic potential.
巻・号 3(6)
ページ 57
公開日 2012-12-19
DOI 10.1186/scrt148
PII scrt148
PMID 23253356
PMC PMC3580487
MeSH ATP Binding Cassette Transporter, Subfamily G, Member 2 ATP-Binding Cassette Transporters / genetics ATP-Binding Cassette Transporters / metabolism Antigens, CD / metabolism Biomarkers / metabolism Cell Differentiation Cells, Cultured Fetal Blood / cytology* Homeodomain Proteins / genetics Homeodomain Proteins / metabolism Humans Immunophenotyping Mesenchymal Stem Cells / cytology* Mesenchymal Stem Cells / metabolism Nanog Homeobox Protein Neoplasm Proteins / genetics Neoplasm Proteins / metabolism Neurons / cytology* Neurons / metabolism Octamer Transcription Factor-3 / genetics Octamer Transcription Factor-3 / metabolism Pluripotent Stem Cells / cytology SOXB1 Transcription Factors / genetics SOXB1 Transcription Factors / metabolism
IF 5.116
リソース情報
ヒト・動物細胞 UCB408E6E7TERT-33(RCB2080)