論文 - 詳細
| RRC ID | 62148 |
|---|---|
| 著者 | Pai R, Cover TL, Tarnawski AS. |
| タイトル | Helicobacter pylori vacuolating cytotoxin (VacA) disorganizes the cytoskeletal architecture of gastric epithelial cells. |
| ジャーナル | Biochem Biophys Res Commun |
| Abstract |
Helicobacter pylori colonization of the gastric mucosa induces peptic ulcer disease and interferes with ulcer healing. Re-epithelialization is an essential component of ulcer healing. It requires cell migration and proliferation which are dependent on the cell cytoskeleton. Most H. pylori strains produce a toxin (VacA) that induces multiple structural and functional changes in epithelial cells. In this study, we investigated the effects of VacA on the gastric epithelial cell cytoskeletal architecture. Exposure of rat gastric epithelial cells to purified VacA from H. pylori 60190 significantly inhibited actin stress fiber formation (83 +/- 5% reduction; p < 0.0001) and disorganized microtubule pattern (90 +/- 8%; p < 0.001). Furthermore, VacA treatment significantly reduced tyrosine phosphorylation of focal adhesion kinase (FAK) (by 45 +/- 6%; p < 0.002) and its expression in focal adhesions (73 +/- 8%; p < 0.0001). These findings suggest that H. pylori VacA interferes with cytoskeleton-dependent cell functions and with the transmission of signals related to cell spreading and growth. |
| 巻・号 | 262(1) |
| ページ | 245-50 |
| 公開日 | 1999-8-19 |
| DOI | 10.1006/bbrc.1999.1194 |
| PII | S0006-291X(99)91194-4 |
| PMID | 10448099 |
| MeSH | Actin Cytoskeleton / drug effects Actin Cytoskeleton / metabolism Actins / metabolism Animals Bacterial Proteins / pharmacology* Cell Adhesion Cell Adhesion Molecules / metabolism Cell Line Cell Membrane / drug effects Cell Membrane / metabolism Centrosome / drug effects Centrosome / metabolism Cytoskeleton / drug effects* Cytoskeleton / metabolism Focal Adhesion Kinase 1 Focal Adhesion Protein-Tyrosine Kinases Gastric Mucosa / cytology Gastric Mucosa / drug effects* Gastric Mucosa / enzymology Gastric Mucosa / metabolism Helicobacter pylori Intermediate Filaments / drug effects Intermediate Filaments / metabolism Microtubules / drug effects Microtubules / metabolism Peptic Ulcer / microbiology Phosphorylation / drug effects Phosphotyrosine / metabolism Protein-Tyrosine Kinases / metabolism Rats Tubulin / metabolism |
| IF | 2.985 |
| リソース情報 | |
| ヒト・動物細胞 | RGM1(RCB0876) |