論文 - 詳細
| RRC ID | 62165 |
|---|---|
| 著者 | Doi K, Horiuchi T, Uchinami M, Tabo T, Kimura N, Yokomachi J, Yoshida M, Tanaka K. |
| タイトル | Hepatic ischemia-reperfusion promotes liver metastasis of colon cancer. |
| ジャーナル | J Surg Res |
| Abstract |
BACKGROUND:The effects of hepatic ischemia/reperfusion (I/R) on liver metastasis have not been fully examined. We examined hepatic I/R and liver metastasis of colorectal cancer in a rat model; we also quantitated expression of E-selectin (ELAM-1) mRNA after I/R. MATERIALS AND METHODS:Rats underwent 30 or 60 min of 70% partial hepatic ischemia. After 60 min of reperfusion, rat colon adenocarcinoma cells (RCN-H4) were inoculated intrasplenically. The number of tumor nodules on the liver surface was determined 3 weeks later. Expression of E-selectin mRNA was determined at 1, 3, and 6 h after ischemia by quantitative RT-PCR. RESULTS:Hepatic I/R promoted liver metastasis of RCN-H4 and induced the expression of E-selectin mRNA in both clamped and unclamped liver lobes. The number of tumor nodules and the expression of E-selectin mRNA after 60 min of ischemia was greater than that after 30 min. CONCLUSIONS:Hepatic I/R, especially with a long duration of ischemia, induces expression of E-selectin and promotes liver metastasis of colon cancer in rats. |
| 巻・号 | 105(2) |
| ページ | 243-7 |
| 公開日 | 2002-6-15 |
| DOI | 10.1006/jsre.2002.6356 |
| PII | S0022480402963560 |
| PMID | 12121713 |
| MeSH | Animals Colonic Neoplasms / pathology* E-Selectin / genetics Ischemia / complications* Liver / metabolism Liver Circulation* Liver Neoplasms / etiology* Liver Neoplasms / secondary* Male RNA, Messenger / metabolism Rats Rats, Inbred F344 Reperfusion Injury / complications* |
| IF | 1.841 |
| リソース情報 | |
| ヒト・動物細胞 | RCN-9(RCB0511) |