論文 - 詳細
| RRC ID | 62210 |
|---|---|
| 著者 | Li Z, Yamauchi Y, Kamakura M, Murayama T, Goshima F, Kimura H, Nishiyama Y. |
| タイトル | Herpes simplex virus requires poly(ADP-ribose) polymerase activity for efficient replication and induces extracellular signal-related kinase-dependent phosphorylation and ICP0-dependent nuclear localization of tankyrase 1. |
| ジャーナル | J Virol |
| Abstract |
Tankyrase 1 is a poly(ADP-ribose) polymerase (PARP) which localizes to multiple subcellular sites, including telomeres and mitotic centrosomes. Poly(ADP-ribosyl)ation of the nuclear mitotic apparatus (NuMA) protein by tankyrase 1 during mitosis is essential for sister telomere resolution and mitotic spindle pole formation. In interphase cells, tankyrase 1 resides in the cytoplasm, and its role therein is not well understood. In this study, we found that herpes simplex virus (HSV) infection induced extensive modification of tankyrase 1 but not tankyrase 2. This modification was dependent on extracellular signal-regulated kinase (ERK) activity triggered by HSV infection. Following HSV-1 infection, tankyrase 1 was recruited to the nucleus. In the early phase of infection, tankyrase 1 colocalized with ICP0 and thereafter localized within the HSV replication compartment, which was blocked in cells infected with the HSV-1 ICP0-null mutant R7910. In the absence of infection, ICP0 interacted with tankyrase 1 and efficiently promoted its nuclear localization. HSV did not replicate efficiently in cells depleted of both tankyrases 1 and 2. Moreover, XAV939, an inhibitor of tankyrase PARP activity, decreased viral titers to 2 to 5% of control values. We concluded that HSV targets tankyrase 1 in an ICP0- and ERK-dependent manner to facilitate its replication. |
| 巻・号 | 86(1) |
| ページ | 492-503 |
| 公開日 | 2012-1-1 |
| DOI | 10.1128/JVI.05897-11 |
| PII | JVI.05897-11 |
| PMID | 22013039 |
| PMC | PMC3255871 |
| MeSH | Animals Cell Line Cell Nucleus / enzymology* Cell Nucleus / genetics Extracellular Signal-Regulated MAP Kinases / genetics Extracellular Signal-Regulated MAP Kinases / metabolism* Herpes Simplex / enzymology* Herpes Simplex / genetics Herpes Simplex / virology Herpesvirus 1, Human / enzymology Herpesvirus 1, Human / genetics Herpesvirus 1, Human / physiology* Humans Immediate-Early Proteins / genetics Immediate-Early Proteins / metabolism* Phosphorylation Protein Binding Protein Transport Tankyrases / genetics Tankyrases / metabolism* Ubiquitin-Protein Ligases / genetics Ubiquitin-Protein Ligases / metabolism* Virus Replication* |
| IF | 4.501 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 12 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | Vero(RCB0001) HEp-2(RCB1889) |