RRC ID 62237
Author Ozeki N, Hase N, Hiyama T, Yamaguchi H, Kawai R, Kondo A, Nakata K, Mogi M.
Title IL-1β-induced, matrix metalloproteinase-3-regulated proliferation of embryonic stem cell-derived odontoblastic cells is mediated by the Wnt5 signaling pathway.
Journal Exp Cell Res
Abstract We previously established a method for differentiating induced pluripotent stem cells and embryonic stem (ES) cells into α2 integrin-positive odontoblast-like cells. We also reported that interleukin (IL)-1β induces matrix metalloproteinase (MMP)-3-regulated cell proliferation and suppresses apoptosis in these cells, suggesting that MMP-3 plays a potentially unique physiological role in the regeneration of odontoblast-like cells. Here, we examined whether up-regulation of MMP-3 activity by IL-1β was mediated by Wnt signaling and led to increased proliferation of odontoblast-like cells. IL-1β increased mRNA and protein levels of Wnt5a, Wnt5b and the Wnt receptor Lrp5. Exogenous Wnt5a and Wnt5b were found to increase MMP-3 mRNA, protein and activity, and interestingly the rate of proliferation in these cells. Treatment with siRNAs against Wnt5a, Wnt5b and Lrp5 suppressed the IL-1β-induced increase in MMP-3 expression and suppressed cell proliferation, an effect rescued by application of exogenous Wnt5. These results demonstrate the sequential involvement of Wnt5, Lrp5 and MMP-3 in effecting IL-1β-induced proliferation of ES cell-derived odontoblast-like cells.
Volume 328(1)
Pages 69-86
Published 2014-10-15
DOI 10.1016/j.yexcr.2014.05.006
PII S0014-4827(14)00197-9
PMID 24851717
MeSH Animals Apoptosis Blotting, Western Cell Proliferation* Cells, Cultured Embryonic Stem Cells / cytology* Embryonic Stem Cells / metabolism Low Density Lipoprotein Receptor-Related Protein-5 / antagonists & inhibitors Low Density Lipoprotein Receptor-Related Protein-5 / genetics Low Density Lipoprotein Receptor-Related Protein-5 / metabolism Matrix Metalloproteinase 3 / genetics Matrix Metalloproteinase 3 / metabolism* Mice Odontoblasts / cytology* Odontoblasts / metabolism RNA, Messenger / genetics Real-Time Polymerase Chain Reaction Reverse Transcriptase Polymerase Chain Reaction Wnt Proteins / antagonists & inhibitors Wnt Proteins / genetics Wnt Proteins / metabolism* Wnt-5a Protein
IF 3.383
Human and Animal Cells B6G-2(AES0003)