| RRC ID |
62249
|
| 著者 |
Li QC, Li HJ, Liu S, Liang Y, Wang X, Cui L.
|
| タイトル |
Inhibition of γ-secretase by retinoic acid chalcone (RAC) induces G2/M arrest and triggers apoptosis in renal cell carcinoma.
|
| ジャーナル |
Int J Clin Exp Pathol
|
| Abstract |
The present study was devised to investigate the effect of RAC on inhibition of cell proliferation and apoptosis of renal carcinoma cells. MTT assay and flow cytometry analysis were used to determine cell proliferation and apoptosis along with cell cycle examination. Western blot analysis and immunohistochemistry were used for the detection of expression levels of Notch1 and Jagged1 in renal cell carcinoma (RCC) and normal kidney tissues. The results revealed a significant inhibition of cell proliferation, G2/M phase cell cycle arrest and cell apoptosis at 30 μM concentration of RAC after 72 h. In ACHN and 769-P cells, the population in G2/M phase was increased to 45.27, and 54.23% respectively on treatment with 30 μM RAC for 72 h. In 769-P and ACHN renal carcinoma cells treatment with 30 μM RAC caused 69.71 and 59.27% of the cells to undergo apoptosis compared to 5.23 and 4.93% respectively in control cells. The positive staining rates of Notch1 and Jagged1 in renal carcinoma tissues were 95.3 and 93.0% compared to normal kidney tissues 36.4 and 42.4% respectively. Treatment of renal carcinoma tissues caused a significant decrease in staining rates of Notch1 and Jagged1 after 96 h. Thus RAC can be a potent agent in the treatment of renal cell carcinoma.
|
| 巻・号 |
8(3)
|
| ページ |
2400-7
|
| 公開日 |
2015-1-1
|
| PMID |
26045747
|
| PMC |
PMC4440056
|
| MeSH |
Adult
Aged
Aged, 80 and over
Amyloid Precursor Protein Secretases / antagonists & inhibitors*
Amyloid Precursor Protein Secretases / metabolism
Antineoplastic Agents / pharmacology*
Apoptosis / drug effects*
Calcium-Binding Proteins / metabolism
Carcinoma, Renal Cell / drug therapy*
Carcinoma, Renal Cell / enzymology
Carcinoma, Renal Cell / pathology
Cell Line, Tumor
Cell Proliferation / drug effects
Chalcones / pharmacology*
Dose-Response Relationship, Drug
Down-Regulation
Female
G2 Phase Cell Cycle Checkpoints / drug effects*
Humans
Intercellular Signaling Peptides and Proteins / metabolism
Jagged-1 Protein
Kidney Neoplasms / drug therapy*
Kidney Neoplasms / enzymology
Kidney Neoplasms / pathology
Male
Membrane Proteins / metabolism
Middle Aged
Receptor, Notch1 / metabolism
Serrate-Jagged Proteins
Signal Transduction / drug effects
Time Factors
Tretinoin / analogs & derivatives
Tretinoin / pharmacology*
Young Adult
|
| IF |
0.252
|
| リソース情報 |
| ヒト・動物細胞 |
TUHR14TKB(RCB1383) |
