論文 - 詳細
| RRC ID | 6229 |
|---|---|
| 著者 | Caron SJ, Prober D, Choy M, Schier AF. |
| タイトル | In vivo birthdating by BAPTISM reveals that trigeminal sensory neuron diversity depends on early neurogenesis. |
| ジャーナル | Development |
| Abstract |
Among sensory systems, the somatic sense is exceptional in its ability to detect a wide range of chemical, mechanical and thermal stimuli. How this sensory diversity is established during development remains largely elusive. We devised a method (BAPTISM) that uses the photoconvertible fluorescent protein Kaede to simultaneously analyze birthdate and cell fate in live zebrafish embryos. We found that trigeminal sensory ganglia are formed from early-born and late-born neurons. Early-born neurons give rise to multiple classes of sensory neurons that express different ion channels. By contrast, late-born neurons are restricted in their fate and do not form chemosensory neurons expressing the ion channel TrpA1b. Accordingly, larvae lacking early-born neurons do not respond to the TrpA1b agonist allyl isothiocyanate. These results indicate that the multimodal specification and function of trigeminal sensory ganglia depends on the timing of neurogenesis. |
| 巻・号 | 135(19) |
| ページ | 3259-69 |
| 公開日 | 2008-10-1 |
| DOI | 10.1242/dev.023200 |
| PII | dev.023200 |
| PMID | 18755773 |
| PMC | PMC2596952 |
| MeSH | Animals Animals, Genetically Modified Gene Expression Regulation, Developmental Ion Channels / genetics Ion Channels / metabolism Luminescent Proteins / genetics Luminescent Proteins / metabolism Models, Neurological Neurogenesis* / genetics Neurogenesis* / physiology Recombinant Proteins / genetics Recombinant Proteins / metabolism Sensory Receptor Cells / cytology* Sensory Receptor Cells / metabolism TRPA1 Cation Channel Time Factors Transient Receptor Potential Channels Trigeminal Ganglion / cytology Trigeminal Ganglion / embryology* Trigeminal Ganglion / metabolism Zebrafish / embryology* Zebrafish / genetics Zebrafish / metabolism Zebrafish Proteins / genetics Zebrafish Proteins / metabolism |
| IF | 5.611 |
| 引用数 | 43 |
| WOS 分野 | DEVELOPMENTAL BIOLOGY |
| リソース情報 | |
| ゼブラフィッシュ | rw0130a (Tg (Huc:Kaede)) |