| RRC ID |
62547
|
| 著者 |
Ning X, Sun S, Hong L, Liang J, Liu L, Han S, Liu Z, Shi Y, Li Y, Gong W, Zhang S, Chen Y, Guo X, Cheng Y, Wu K, Fan D.
|
| タイトル |
Calcyclin-binding protein inhibits proliferation, tumorigenicity, and invasion of gastric cancer.
|
| ジャーナル |
Mol Cancer Res
|
| Abstract |
Calcyclin-binding protein/Siah-1-interacting protein (CacyBP/SIP), a target protein of the S100 family, which includes S100A6, S100A1, S100A12, S100B, and S100P, has been identified as a component of a novel ubiquitinylation complex leading to beta-catenin degradation. However, the function of CacyBP/SIP in gastric cancer has not been elucidated. In the present study, we prepared CacyBP/SIP overexpressing and knockdown cell lines of gastric cancer. Forced CacyBP/SIP expression inhibited the proliferation of gastric cancer cells, suppressed tumorigenicity in vitro, and prolonged the survival time of tumor-bearing nude mice. In addition, increased CacyBP/SIP repressed the invasive potential of gastric cancer cells. Conversely, the down-regulation of CacyBP/SIP by RNA interference showed the opposite effects. Further studies showed that depressed CacyBP/SIP increased the expression of total and nuclear beta-catenin at the protein level and elevated the transcriptional activity of Tcf/LEF. Taken together, our results suggest that CacyBP/SIP may be a potential inhibitor of cell growth and invasion in the gastric cancer cell, at least in part through the effect on beta-catenin protein expression and transcriptional activation of Tcf/LEF.
|
| 巻・号 |
5(12)
|
| ページ |
1254-62
|
| 公開日 |
2007-12-1
|
| DOI |
10.1158/1541-7786.MCR-06-0426
|
| PII |
5/12/1254
|
| PMID |
18171983
|
| MeSH |
Animals
Calcium-Binding Proteins / genetics
Calcium-Binding Proteins / metabolism*
Cell Division / physiology
Cell Line, Tumor
Down-Regulation / physiology
Gene Expression Regulation, Neoplastic
Humans
Lymphoid Enhancer-Binding Factor 1 / metabolism
Mice
Mice, Inbred BALB C
Mice, Nude
Neoplasm Invasiveness
Neoplasm Transplantation
RNA, Small Interfering
Stomach Neoplasms / metabolism*
Stomach Neoplasms / pathology*
Transcriptional Activation / physiology
Transfection
Up-Regulation / physiology
beta Catenin / genetics
beta Catenin / metabolism
|
| IF |
4.63
|
| リソース情報 |
| ヒト・動物細胞 |
MKN28(RCB1000)
MKN45(RCB1001) |
