RRC ID 62704
Author Trivedi D, Cm V, Bisht K, Janardan V, Pandit A, Basak B, H S, Ramesh N, Raghu P.
Title A genome engineering resource to uncover principles of cellular organization and tissue architecture by lipid signaling.
Journal Elife
Abstract Phosphoinositides (PI) are key regulators of cellular organization in eukaryotes and genes that tune PI signaling are implicated in human disease mechanisms. Biochemical analyses and studies in cultured cells have identified a large number of proteins that can mediate PI signaling. However, the role of such proteins in regulating cellular processes in vivo and development in metazoans remains to be understood. Here, we describe a set of CRISPR-based genome engineering tools that allow the manipulation of each of these proteins with spatial and temporal control during metazoan development. We demonstrate the use of these reagents to deplete a set of 103 proteins individually in the Drosophila eye and identify several new molecules that control eye development. Our work demonstrates the power of this resource in uncovering the molecular basis of tissue homeostasis during normal development and in human disease biology.
Volume 9
Published 2020-12-15
DOI 10.7554/eLife.55793
PII 55793
PMID 33320085
PMC PMC7771963
MeSH Animals CRISPR-Cas Systems / genetics* Clustered Regularly Interspaced Short Palindromic Repeats / genetics* Drosophila melanogaster / embryology Drosophila melanogaster / genetics* Eye / embryology* Eye / metabolism Gene Editing / methods Gene Knockout Techniques Genetic Engineering / methods* Genome, Insect / genetics Lipid Metabolism Phosphatidylinositols / metabolism* RNA, Guide, Kinetoplastida / biosynthesis RNA, Guide, Kinetoplastida / genetics Sequence Deletion / genetics Signal Transduction / physiology
IF 7.08
Drosophila pBFvU6.2 pBFvU6.2B