| RRC ID |
62756
|
| 著者 |
Cui M, Sun J, Hou J, Fang T, Wang X, Ge C, Zhao F, Chen T, Xie H, Cui Y, Yao M, Li J, Li H.
|
| タイトル |
The suppressor of cytokine signaling 2 (SOCS2) inhibits tumor metastasis in hepatocellular carcinoma.
|
| ジャーナル |
Tumour Biol
|
| Abstract |
Hepatocellular carcinoma (HCC) is a common cause of cancer-related death worldwide, and its incidence continues to increase. However, the mechanism underlying the development and progression of HCC remains unknown. The suppressor of cytokine signaling 2 (SOCS2) is a member of the SOCS family and influences the carcinogenesis of multiple types of tumors, but the biological roles of SOCS2 in HCC remain unclear. In this study, we found that SOCS2 expression was reduced in HCC tissues compared with matched noncancerous liver tissues. Moreover, decreased SOCS2 expression was significantly associated with the presence of intrahepatic metastasis and high histological grade in HCC patients. Colony formation assays and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assays demonstrated that overexpression of SOCS2 or knockdown of endogenous SOCS2 did not significantly affect cell proliferation and tumorigenicity in HCC cells in vitro and in vivo. However, SOCS2 overexpression significantly inhibited the migration and invasion of HCC cells in vitro and inhibited metastasis in vivo. Consistent with these findings, the knockdown of endogenous SOCS2 enhanced migration and invasion in HCC cells in vitro. Our study demonstrated that SOCS2 inhibited human HCC metastasis, and SOCS2 might provide a new potential therapeutic strategy for treating HCC.
|
| 巻・号 |
37(10)
|
| ページ |
13521-13531
|
| 公開日 |
2016-10-1
|
| DOI |
10.1007/s13277-016-5215-7
|
| PII |
10.1007/s13277-016-5215-7
|
| PMID |
27465557
|
| MeSH |
Adult
Aged
Aged, 80 and over
Animals
Apoptosis
Biomarkers, Tumor / genetics
Biomarkers, Tumor / metabolism*
Blotting, Western
Carcinoma, Hepatocellular / metabolism
Carcinoma, Hepatocellular / secondary*
Carcinoma, Hepatocellular / surgery
Cell Movement
Cell Proliferation
Female
Follow-Up Studies
Gene Expression Regulation, Neoplastic*
Humans
Immunoenzyme Techniques
Liver Neoplasms / metabolism
Liver Neoplasms / pathology*
Liver Neoplasms / surgery
Lymphatic Metastasis
Male
Mice
Mice, Inbred BALB C
Mice, Nude
Middle Aged
Neoplasm Grading
Neoplasm Invasiveness
Prognosis
RNA, Messenger / genetics
Real-Time Polymerase Chain Reaction
Reverse Transcriptase Polymerase Chain Reaction
Suppressor of Cytokine Signaling Proteins / genetics
Suppressor of Cytokine Signaling Proteins / metabolism*
Survival Rate
Tumor Cells, Cultured
Xenograft Model Antitumor Assays
Young Adult
|
| IF |
3.65
|
| リソース情報 |
| ヒト・動物細胞 |
HuH-7 |
