論文 - 詳細
| RRC ID | 62841 |
|---|---|
| 著者 | Kurita H, Haruta N, Uchihashi Y, Seto T, Takashima K. |
| タイトル | Strand breaks and chemical modification of intracellular DNA induced by cold atmospheric pressure plasma irradiation. |
| ジャーナル | PLoS One |
| Abstract |
DNA damage in the A549 human lung cancer cell line treated with cold plasma irradiation was investigated. We confirmed that cold atmospheric plasma generated reactive oxygen and nitrogen species (RONS) in a liquid, and the intracellular RONS level was increased in plasma-irradiated cells. However, a notable decrease in cell viability was not observed 24 hours after plasma irradiation. Because RONS induce oxidative damage in cells, strand breaks and chemical modification of DNA in the cancer cells were investigated. We found that 8-oxoguanine (8-oxoG) formation as well as DNA strand breaks, which have been thoroughly investigated, were induced by plasma irradiation. In addition, up-regulation of 8-oxoG repair enzyme was observed after plasma irradiation. |
| 巻・号 | 15(5) |
| ページ | e0232724 |
| 公開日 | 2020-1-1 |
| DOI | 10.1371/journal.pone.0232724 |
| PII | PONE-D-20-06283 |
| PMID | 32374749 |
| PMC | PMC7202611 |
| MeSH | A549 Cells Cell Survival / drug effects DNA / metabolism DNA Breaks, Double-Stranded / drug effects* DNA Breaks, Single-Stranded / drug effects* DNA Glycosylases / metabolism* DNA Repair* Guanine / analogs & derivatives* Guanine / biosynthesis Humans Oxidative Stress / drug effects Plasma Gases / pharmacology* Reactive Nitrogen Species / metabolism Reactive Oxygen Species / metabolism Up-Regulation |
| IF | 2.74 |
| リソース情報 | |
| ヒト・動物細胞 | A549(RCB0098) |